Cynomolgus Monkey Small Intestinal Microvascular Endothelial Cells from Creative Bioarray are isolated from Cynomolgus monkey small intestinal tissue. Cynomolgus Monkey Small Intestinal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
Cynomolgus Monkey Small Intestinal Microvascular Endothelial Cells from Creative Bioarray display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody CD31 (Catalog No. ab9498, Abcam), VE-cadherin Antibody (Catalog No. sc-28644, Santa Cruz), or VE-cadherin (Catalog No. ALX-210-232-C100, Enzo Life Sciences, Inc.) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded on a plate ready for experiments under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
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