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Invasion and metastasis are responsible for the high morbidity and mortality in cancer patients. Invasion into the surrounding normal tissues is generally considered to be a key hallmark of malignant tumors. More efforts for anticancer drug development now move towards therapies that targeted on the inhibition of this key "hallmark" of cancer. The 3D tumor spheroid invasion assay allows cells to invade out of the tumor mass into the extracellular matrix-like environment. The true 3D manner takes into account important aspects of the pathophysiology of a tumor mass, in which the tumor spheroids may experience hypoxia and nutrient deprivation which may cause the change in gene expression and promote migration and invasion. Creative Bioarray believes that the rapid, automatable 3D invasion system that enables highly reproducible experimental conditions and high throughput analysis will give our customers the best results to identify novel therapeutic agents.
Creative Bioarray 3D invasion assay service advantages:
3D tumor spheroids invasion assays: Multicellular spheroids are embedded into 3D ECM. It is expected to see that non-invasive cancer cells stay as compact spheroids with a distinct border to the surrounding ECM and do not show any obvious signs of invasion. However, invasive cells or endothelial cells start to invade into the surrounding matrix and display outgrowth from the spheroids.
Figure 1 The representative images for 3D invasion assay for glioblastoma spheroids
Figure 2 The representative image analysis of 3D invasion assay for glioblastoma spheroids
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We also provide 2D cell migration and invasion service for our customers