Telomere Length Analysis (qPCR assay)

Telomeres represent the nucleotide repeat sequences at the ends of chromosomes and are important for chromosome stability. They can shorten at each round of DNA replication mainly because of incomplete DNA synthesis of the lagging strand. Reduced relative telomere length is associated with aging and a range of disease states.

Creative Bioarray employs qPCR assay to measure absolute telomere length, which is a simple and reproducible method. The primers, conceived to create a brief, fixed-length product, can attach only one fluorescent group to each amplicon produced in the experiment, distinguishably different from the mentioned SYBR green-based methods. We achieve this by using our distinctive primers for both telomeric repeat amplification and the amplification of a single-copy gene reference sequence. Consequently, this leads to elongated linear ranges for our calibration curves used in this technique and a more precise length determination for shorter telomeres.

Features:

• Human Telomere Length Analysis Service, Mouse Telomere Length Analysis Service, Rat Telomere Length Analysis Service, Monkey Telomere Length Analysis Service, Rare Species Telomere Length Analysis Service
• High accuracy and sensitivity
• Fast turnaround time
• Competitive pricing

Creative Bioarray offers Telomere Length Analysis (qPCR assay) for your scientific research as follows:

• Sample preparation
• Perform qPCR assay
• Data analysis
• Delivery of the data via e-mail

Quotation and ordering

Our customer service representatives are available 24hr a day! We thank you for choosing Creative Bioarray at your preferred Telomere Length Analysis (qPCR assay) Service.

References

1. Blackburn E, Gall J. A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena. Journal of molecular biology. 1978;120(1):33–53. pmid:642006
2. Moyzis R, Buckingham J, Cram L, Dani M, Deaven L, Jones M, et al. A highly conserved repetitive DNA sequence,(TTAGGG) n, present at the telomeres of human chromosomes. Proceedings of the National Academy of Sciences. 1988;85(18):6622–6. pmid:3413114
3. Allshire RC, Dempster M, Hastie ND. Human telomeres contain at least three types of G-rich repeat distributed non-randomly. Nucleic Acids Res. 1989;17(12):4611–27. pmid:2664709
4. Therkelsen A, Nielsen A, Koch J, Hindkjaer J, Kølvraa S. Staining of human telomeres with primed in situ labeling (PRINS). Cytogenet Cell Genet. 1995;68(1–2):115–8. Epub 1995/01/01. pmid:7525160.
5. Feuerbach L, Sieverling L, Deeg K, Ginsbach P, Hutter B, Buchhalter I, et al. TelomereHunter–in silico estimation of telomere content and composition from cancer genomes. BMC bioinformatics. 2019;20(1):1–11. pmid:30606105
6. Morinha F, Magalhães P, Blanco G. Standard guidelines for the publication of telomere qPCR results in evolutionary ecology. Molecular Ecology Resources. 2020;20(3). pmid:32133733
7. Bustin S, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, et al. The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments. Clinical Chemistry. 2009;55(4):611–22. pmid:19246619