NZB/W F1 Mouse Model
The NZB/W F1 mouse model of spontaneous systemic lupus erythematosus (SLE) is a model of SLE which was most commonly used. NZB/W F1 mouse develops severe lupus-like disease characterized by gender bias, splenomegaly, lymphadenopathy and elevated serum Anti-nuclear Antibodies (ANA) mostly directed against DNA. In addition, immune complex-mediated nephritis develops by 5-6 months of age, resulting in kidney failure and death at about 12 months of age. Disease is more severe in females, in part, due to high estrogen levels. Male NZBWF1 mice die at an average of 58 weeks of age and females at 36 weeks. Creative Bioarray specializes in providing customized pharmacodynamic research services to help customers assess the efficacy of drug candidates and study the associated pathological mechanisms of SLE through NZB/W F1 mouse model.
Our capabilities
- We detect autoantibodies in animal serum by ELISA, Immunofluorescence, etc.
- We evaluate various cytokines through IHC, ELISA, etc.
- We identify Immunocyte subsets in spleens by flow cytometry.
Assays available
- PK/PD blood collections
- Quantitative detection of Anti-dsDNA and total IgG by ELISA
- Clinical chemistry analysis
- Histopathological evaluation
- Cytokine/chemokine analysis
- Flow cytometry in lymph nodes, spleen or bone marrow
- Organ weights (kidneys, spleen, lymph nodes)
- Survival rate
With extensive experience in the field of SLE, we are confident to help you to overcome any upcoming challenges. Our experts are fully capable of customizing our protocols and assays to meet your specific needs. With our help, we wish to facilitate your research with high efficiency.
Study examples
Figure. 1. A GpG-ODN delays the development of proteinuria in NZB/W mice.
Figure. 2. CpG-ODN and GpG-ODN treatment modulates NZB/W mouse T lymphocyte responses ex vivo.
Quotation and ordering
If you have any special needs or questions regarding our services, please feel free to contact us. We look forward to cooperating with you in the future.
Reference
Graham K L et al. Treatment with a Toll-like receptor inhibitory GpG oligonucleotide delays and attenuates lupus nephritis in NZB/W mice[J]. Autoimmunity, 2010, 43(2):140-155.
