Digital ISH Image Quantification and Statistical Analysis

In situ hybridization (ISH, CISH, FISH) is a popular approach for the evaluation of changes in biomarker expression or a particular drug response effect in tissues, especially in the case of novel biomarkers where robust, well validated antibodies are not commercially available. And investigating RNA at a single cell level can give important information about a cell’s dynamic gene expression. When analyzed in situ and in the context of the whole tissue, researchers can identify aberrant gene expression associated with disease states, such as cancer and neurological disease.

DNA FISH analysis is also available in Creative Bioarray include the FISH Amplification & Deletion Analysis module, used to quantify up to two fluorescently labeled DNA probes to measure amplification or deletion, and the FISH Break apart & Fusion Analysis module, used to measure gene rearrangements detected using break-apart fusion probes.

Fig. 1 AN RNA ISH assay of a non-small-cell lung carcinoma, probed for the immune checkpoint markers PD-L1 (green) and CTLA4 (red) (A). Our system mark-up images are shown in (B) and (C) which visualize where the ISH module has detected probes (B) and where cells have been detected (C). Orange and red dots shown in (B) represent single and clusters of red probes, with the two shades of green dots representing the Expression of PDL-1 and T-lymphocytes identified by high levels of CTLA4 expression (red probe).

Fig 2. A DNA FISH assay for ALK translocation.

Benefits

• Receive highly detailed data quantifying RNA expression/DNA signals on a cell by cell basis across a whole tissue section or within defined regions of interest.
• Our automated batch analysis service objectively analyses each section using the same algorithm to reduce variability and improve data quality and interpretation.

Quotation and ordering

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