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MEC-2

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Cat.No.
CSC-C0511
Description
Established in 1994 from the peripheral blood of a 62-year-old Caucasian man with chronic B cell leukemia (B-CLL in prolymphocytoid transformation to B-PLL) prior to therapy; serial sister cell line of MEC-1
Species
Human
Source
chronic B cell leukemia
Recommended Medium
Culture Properties
suspension
Morphology
polymorphic cells growing in suspension in large clumps; clumps can easily be segregated
Karyotype
Human flat-moded hypertriploid karyotype with 7% polyploidy - 75-80<3n>XX/XXY, -6, -9, -10, +11, +12, -13, +15, -17, -21, +10mar - add(X)(p22)x2, inv(12)(?p12.2?q24.1)x2, add(18)(q2?1) - karyotypically distinct from MEC-1 (as reported)
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -
Viruses: PCR: EBV +, HBV -, HCV -, HIV
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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