Description
Derived from a Burkitt's lymphoma which does not possess the EBV genome. EBV infectability and permanent conversion into EBV positive sub-lines is possible by in vitro infection. The cells have B lymphocyte characteristics, with surface associated mu and kappa chains.
Organism
Homo sapiens, human
STR DNA Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 13,14
D16S539: 10,13
D5S818: 7,12
D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 15,16
Karyotype
Human hypodiploid karyotype; 45(44-46)<2n>X, -Y, del(2)(p16p23), t(8;14)(q24;q32.2), add(14)(p11), der(16)t(7;16)(q11.2;p13.2), der(17)t(13;17)(q33;p11.3)t(13;3)(q13;q?25), del(20)(q12); carries t(8;14) effecting IGH@-MYC rearrangement
Culture Properties
Suspension
Quality Control
Mycoplasma: contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cy
Storage and Shipping
liquid nitrogen vapor phase
Disease
a Burkitt's lymphoma
Citation Guidance
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USA: 1-631-626-9181
Europe: 44-207-097-1828
