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CAL-33

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Cat.No.
CSC-C0479
Description
Established from the surgically removed fragment of a tongue lesion from a 69-year-old man with moderately differentiated squamous cell carcinoma of the tongue in 1983 (prior to therapy)
Organism
Human
Karyotype
Human hyperdiploid karyotype with 4% polyploidy 49(45-49)<2n>XY/XYY, +7, +9, -18, +20, +mar, add(X)(p22), add(3)(p22-24), i(7p), i(8q), add(9)(p24), del(9)(p2?2) - resembles published karyotype
Source
tongue squamous cell carcinoma
Culture Properties
monolayer
Morphology
epitheloid cells growing adherent in monolayers
Recommended Medium
90% DMEM + 10% h.i. FBS
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CM-1010X
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