HEP-3B

Cat.No.: CSC-C0245

Species: Homo sapiens, human

Source: Liver

Morphology: epithelial-like cells growing as monolayers

Culture Properties: Monolayer

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C0245

Description

Established from the tumor tissue of an 8-year-old black boy in 1976.The cells contain a 2.3 kb integrated hepatitis B virus genome fragment. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. Cells were described to produce a variety of proteins, e.g. alpha-fetoprotein, albumin, transferrin, alpha2-macroglobulin, alpha1-antitrypsin, haptoglobin and others.

Species

Homo sapiens, human

Source

Liver

Recommended Medium

EMEM + 10% h.i. FBS

Culture Properties

Monolayer

Morphology

epithelial-like cells growing as monolayers

STR DNA Profile

Amelogenin: X
CSF1PO: 8
D13S317: 12,14
D16S539: 10
D5S818: 13
D7S820: 8,10
THO1: 6,7
TPOX: 9
vWA: 17"

Karyotype

Human hypotriploid stemline with near tetraploid side line (6%) - 63 (60-64)<3n>XX, -Y, -1, -1, -2, -3, -4, -8, -9, -13, +der(11)t(1;11)(q11;q14)x2, der(?15)t(1;?15)(q11;q24), i(7p)

Application

The HEP-3B cell line can be used as a suitable transfection host

Quality Control

Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 (+), cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, e

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x106 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What should I pay attention to when adding PBS in the cell culture process?

Pay attention to the wall to add to the edge of the culture dish, do not add PBS directly to the cells, it is easy to blow up all the cells.

Ask a Question

Average Rating: 5.0 | 1 Scientist has reviewed this product

Helpful

The liver cancer cells provided by Creative Bioarray have been instrumental in our study of cancer progression and drug resistance.

07 Nov 2022

Ease of use
After sales services
Value for money

Write your own review

You May Also Need

CM-1041X

CS-001Y

Hep 3B2.1-7

Description: This line contains an integrated hepatitis B virus genome.

Cat#: CSC-C9187W INQUIRY

View Product

OZ

Description: Human bile duct cell line established from ascites of the tumor patient who had differentiated adenocarcinoma.

Cat#: CSC-C6719J INQUIRY

View Product

HuH-28

Description: Human cell line derived from cholangiocellular carcinoma.

Cat#: CSC-C6345J INQUIRY

View Product

LI90

Description: Ito (fat-storing) cells, stellate-shaped mesenchymal cells that exist in the space of Disse of the liver and contain many fat droplets in cytoplasm.

Cat#: CSC-C6775J INQUIRY

View Product

YSCCC

Description: Human cell line derived from cholangiocellular carcinoma. Cell growth is slow.

Cat#: CSC-C6606J INQUIRY

View Product

GS-HepG2

Description: A subline of HepG2. Expressing abundant glutamine synthetase.

Cat#: CSC-C6373J INQUIRY

View Product

For research use only. Not for any other purpose.