Description
Established from the tumor tissue of an 8-year-old black boy in 1976.The cells contain a 2.3 kb integrated hepatitis B virus genome fragment. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. Cells were described to produce a variety of proteins, e.g. alpha-fetoprotein, albumin, transferrin, alpha2-macroglobulin, alpha1-antitrypsin, haptoglobin and others.
Organism
Homo sapiens, human
STR DNA Profile
Amelogenin: X
CSF1PO: 8
D13S317: 12,14
D16S539: 10
D5S818: 13
D7S820: 8,10
THO1: 6,7
TPOX: 9
vWA: 17"
Karyotype
Human hypotriploid stemline with near tetraploid side line (6%) - 63 (60-64)<3n>XX, -Y, -1, -1, -2, -3, -4, -8, -9, -13, +der(11)t(1;11)(q11;q14)x2, der(?15)t(1;?15)(q11;q24), i(7p)
Culture Properties
Monolayer
Morphology
epithelial-like cells growing as monolayers
Quality Control
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 (+), cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, e
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x106 cells/ampoule; ship in dry ice; store in liquid nitrogen
Application
The HEP-3B cell line can be used as a suitable transfection host
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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