TNBS Induced Colitis Model

50% ethanol enema containing trinitrobenzene sulfonic acid (TNBS) induced colitis is an economical and time saving model and can simulate clinical features of human inflammatory bowel disease, especially Crohn's disease. In this model, ethanol is used to effectively disrupt intestinal barrier and TNBS is used as a contact-sensitizing allergen to couple with high molecular weight proteins to elicit significant immunologic responses. Numbers of studies by using this model have revealed the pathogenesis of TNBS colitis associated with the function of a key susceptibility gene named NOD2 in Crohn's disease.

Creative Bioarray has many years of experience in the field of colitis and provides pharmacodynamic services for customers to help them assess the efficacy of compounds and study the associated pathological mechanisms of colitis through TNBS- induced colitis model.

Our capabilities

• We utilize TNBS induced colitis model to test the efficacy of drug candidates targeting IBD.
• We assess the severity of colitis through clinical analysis including weight loss, diarrhea and occult blood in stool.
• We evaluate various cytokines through IHC, ELISA, etc.

Assays available

• Clinical analysis
• Histopathological evaluation
• Analysis of mRNA and protein levels
• Cytokine/chemokine analysis
• Immunohistochemistry

With extensive experience in the field of IBD, we are confident to help you to overcome any upcoming challenges. Our experts are fully capable of customizing our protocols and assays to meet your specific needs. With our help, we wish to facilitate your research with high efficiency.

Study examples

Figure. 1. CP attenuates inflammation in TNBS-induced colitis. Effects of CP on the disease activity index (A), MPO activity (B) and the length of the colon (C). The differences in (A), (B) and (C) were analyzed using one-way ANOVA followed by Tukey's post hoc tests (* P < 0.05, **P < 0.01).

Figure. 2. The pathological section of the colon tissues from rats in the (A) Control groups, (B) Model group, and (C) CP group, and (D) the histological score. The differences in (D) were analyzed using one-way ANOVA followed by Tukey's post hoc tests (* P < 0.05, ** P < 0.01).

Quotation and ordering

If you have any special needs or questions regarding our services, please feel free to contact us at 631-626-9181 or info@creative-bioarray.com. We look forward to cooperating with you in the future.

Reference

1. Cai Y , et al. Compound polysaccharides ameliorate experimental colitis by modulating gut microbiota composition and function. J Gastroenterol Hepatol, 2018 Dec 27.