In vitro Full Thickness Skin Model

Compared to animal ex­periments or the use of ex vivo skin, in vitro 3D Full Thickness Skin Model (Human Skin Equivalent, FTSK, HSE) used under defined test conditions of­fer several advantages: i) since most models are composed of primary human cells, inter-species extrapolation is avoided; ii) in contrast to ex vivo human skin, repeated application of formulations can be performed for at least several weeks; iii) work with the ready to use epidermal FTSK (HSE) does not require advanced knowledge of cell culture techniques; iv) the employment of FTSK (HSE) leads to the reduction of laboratory animal use in regulatory toxicology as well as in preclinical studies.

Creative Bioarray provides in vitro Full Thickness Skin Model (Human Skin Equivalent, FTSK, HSE) to our customers for developing innovative skin care products, and to cosmetic scientists for evaluating novel ingredients and formulations.

Creative Bioarray Reconstructed FTSK (HSE) are composed of a dermal compartment containing human skin fibroblasts embedded in a collagen matrix and human keratinocytes seeded on top to form the epidermis.

Creative Bioarray Full Thickness Skin Model Feature

• The skin model closest to clinical testing
• Large donor inventory with matched keratinocytes and fibroblasts
• Delivered ready-to-use

Fig.1 Histological examination of FTSK(HSE) model

Specific Markers

• Filaggrin
• Involucrin
• Loricrin
• Corneodesmosin
• Procollagne1
• Tropoelastin
• Decorin
• Perlecan
• Keratin 10
• Keratin 5
• Presence of different epidermal classes of lipids comprising ceramides
• collagen
• Laminin V
• Alpha6Beta4-integrin
• BP antigen
• Ki67
• Others

Fig2. Fluorescence-labeling of different markers in FTSK.

Fig3. Immunol-labeling of different markers in FTSK

Applications

Creative BioarrayFTSK (HSE) can be utilized for the evaluation of toxicity and efficacy of skin therapeutics and cosmetics.

• skin irritation
• skin sensitization
• genotoxicity
• Pigmentation
• skin barrier function & moisturizing
• anti-aging
• stress / inflammation
• UV protection

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Related models

In vitro Reconstructed Human Epidermis (RHE)
Ex vivo Skin Explants
Neurodermatology Skin Model
Pigmented Epidermis Model
Psoriasis Skin Model

For research use only. Not for any other purpose.