Exosome Application

Exosomes derived from diverse cells have therapeutic effects against cellular disorders and organ injury, which are considered as promising agents for diseases therapy. Exosomes contain proteins, RNAs and DNA that could be involved in transmitting signals to recipient cells. Furthermore, they can be crucial vehicles for drug formulations delivery and gene therapy.

Figure 1 Therapeutic application of mesenchymal stromal cell (MSC)-derived exosomes

Exosomes facilitate the delivery of proteins or nucleic acids into target cells. Creative Bioarray develops a reliable protocol to conveniently place plasmid DNA and RNAs (mRNA, siRNA, and miRNA) into isolated exosomes with high efficiency, and subsequently deliver these exosomal species to recipient cells.

Figure 2 Strategy to deliver miRNA-loaded exosomes in vivo and in vitro. Schematic experimental design for delivering miRNA-loaded exosomes.

Figure 3 Characterization of exosomes derived from THP-1 macrophages.

Figure 4 MiR-15a mimics or inhibitors delivered via exosomes were functional in THP-1 macrophages.

Figure 5 Delivery of miR-15a mimic or inhibitor-loaded exosomes to the alveolar macrophage in vivo.

With specialized scientific support, Creative Bioarray offers a complete set of services for exosome application including, but not limited to:

• Exosome Transfection (siRNA, microRNA, mRNA or plasmid DNA)
• Exosome Labeling ( such as RNA, Protein, and Membrane)
• Exosome Targeting (delivering species-loaded exosomes into recipient cells)

  - RNA Packaging into Exosomes
  - Protein Packaging into Exosomes

Creative Bioarray is dedicated to working closely with your team to accelerating your exosome project with the highest quality and the most competitive price. If you are interest in our exosome application service, please let us know what you need and contact us. We look forward to working with you in the future.

Reference

1. Zhang, D.; et al. Enrichment of selective miRNAs in exosomes and delivery of exosomal miRNAs in vitro and in vivo. American journal of physiology-Lung cellular and molecular physiology. 2017, 312(1): L110-L121.