UT-7

Name: UT-7
Brand: Creative Bioarray

Cat.No.: CSC-C0294

Species: Homo sapiens (Human)

Source: Bone Marrow

Morphology: round (relatively large) cells growing singly in suspension

Culture Properties: Suspension

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Scientific Data
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Cat.No.

CSC-C0294

Description

established from the bone marrow of a 64-year-old man with acute myeloid leukemia (AML M7); cells are constitutively cytokine-dependent and responsive to various cytokines

Species

Homo sapiens (Human)

Source

Bone Marrow

Recommended Medium

RPMI 1640 w/o Hepes + 10% FBS +5-7u/ml Epo

Culture Properties

Suspension

Morphology

round (relatively large) cells growing singly in suspension

STR DNA Profile

Amelogenin: X,Y D5S818: 12 D13S317: 8 D7S820: 8 D16S539: 10,12 vWA: 14,18 TH01: 6,9 TPOX: 10 CSF1PO: 12 D19S433: 14,14.2 D21S11: 31.2 D18S51: 14,17 D6S1043: 10,19 D3S1358: 16 Penta D: 9,11 D2S441: 10,11 D8S1179: 11,15 Penta E: 16,17 D12S391: 18,19 D2S1338: 18,23 FGA: 23

Karyotype

human flat-moded hypotetraploid karyotype - 72-88<4n>XXYY, +1, -8, -9, -9, -9, -11, -11, -12, -13, -13, -14, -18, -21, -22, +6mar, del(1)(q42)x2, t(2;4)(p16-23;q27-31)x1-2, der(2)t(2;5)(p16-23;q12-14)x1-2, add(5)(q11), del(5)(q13), der(6;13)(p10;q10), i(7q)x1-2, add(8)(q24)x1-3, del(9)(q22), add(12)(p12-13), der(13)t(13;13)(p11;q14), add(14)(p11), add(15)(p11), add(16)(p1?), add(17)(p11), add(19)(p13)x2, add(19)(q13), i(21q)x1-2 - extensive subclonal variation present - resembles published karyotype

Reference

Publication

Disease

Acute Megakaryoblastic Leukemia

Quality Control

Mycoplasma: negative in PCR assays
Immunology: CD3 -, CD4 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, CD41 (+), CD42 (+), CD68 +, CD235a (+), HLA-DR (+)
Viruses: ELISA: reverse transcriptase negative

Storage and Shipping

ship in dry ice; store in liquid nitrogen

Synonyms

UT7

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

UT-7 is a well-characterized human acute myeloid leukemia cell line established in 1988 from the bone marrow of a 64-year-old male patient with acute megakaryoblastic leukemia. The cells exhibit round morphology, grow predominantly in suspension with approximately 1–2% slight adherence, and possess a hypotetraploid karyotype with extensive subclonal variation.

The defining and most valuable feature of UT-7 is its absolute cytokine dependence for growth and survival. The cells are strictly dependent on interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), or erythropoietin (EPO). This stringent factor dependency renders UT-7 an exceptionally sensitive and quantitative bioassay system for these hematopoietic growth factors.

UT-7 exhibits bipotential differentiation capacity. It can be induced to differentiate along either the erythroid pathway (by EPO) or the megakaryocytic pathway (by phorbol myristate acetate). This plasticity has enabled the derivation of specialized sublines—UT-7/Epo (EPO-dependent), UT-7/GM (GM-CSF-dependent), and UT-7/TPO (thrombopoietin-dependent)—each serving as tailored models for studying specific hematopoietic lineages and signaling pathways.

CD81 Knockdown Inhibits Proliferation and Inducts Apoptosis of AMKL cell line UT-7

CD81 is a cell surface protein that plays an important part in tumor progression. Several studies have shown that CD81 is crucial for cancer cell proliferation, invasion, and metastasis, particularly in leukemia. Su, Narun and Xiaohao Hu hypothesized that CD81 might play a vital role in acute megakaryoblastic leukemia (AMKL). They constructed CD81 knockdown cell line using shRNA and found that CD81 knockout can inhibit the proliferation of AMKL and increase the apoptosis of AMKL in vitro. Therefore, CD81 may be a target of AMKL.

shCD81 significantly upregulated the rate of apoptosis in the UT-7 cell line. — Fig. 1. The rate of apoptosis was upregulated in UT-7 cells with shCD81 (Su, Narun and Xiaohao Hu. 2025).

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