ImmunoFISH Analysis (FISH+IHC)
Creative Bioarray has years of experience in supporting biotechnology and diagnostic partners in the research, and in response to increasing demand for diagnostic and drug co-development services. We now offer the ImmunoFISH Analysis from the assay development, validation and final testing and analysis services for you. Our team is dedicated to developing excellent ImmunoFISH Analysis service for clients.
FISH is considered as a gold standard becaue of its sensitivity and specificity in the detection of any gene expression, long non-coding RNAs for which antibody detection is not possible. But in some types of research, scientists also want to be able to see where proteins are accumulating. So another technique proposed to increase the diagnostic yield of FISH involves adding an immunochemical step to permit simultaneouse analysis of genotype and phenotype on the same tissue sample. The technique called ImmunoFISH was originally developed for the study of metologic malignancies. And the use has since been extended to other neoplastic processes including those of nerve, breast, prostate and the gastrointestinal tract. ImmunoFISH is a combination of a standard immunohistochemical stain with a standard FISH technique, which provides additional information about the asscociation between transcription level, cellular localization, and protein expression in individual cells.
Figure 1. (A) Lung cancer with human PPIB positive control mRNA probe (Red), CAM5.2 acidic cytokeratin (green) with DAPI as counterstain; (B) Human Chromosome X (Red), Y (Yellow) FISH and immunohistochemical stain using anti-von Willebrand factor (Green) on bone marrow transplant
Figure 2. Detection of EBV genomes in plasmablasts/plasma cells and non-B cells in the blood of most patients with EBV lymphoproliferative disorders using Immuno-FISH
Applications of Creative Bioarray’s ImmunoFISH Analysis Service includes but not limited to:
- Demonstrate the colocalization: ImmunoFISH can be used to analyze if the mRNA signal colocalizes with the protein signal, which can demonstrates the colocalization.
- Characterize complex tissue and gene expression: ImmunoFISH can be used to characterize complex tissue structures by using IHC to identify the cell type and ISH to detect gene expression inside these cells.
- Identify secret protein: ImmunoFISH can be used to identify the origin of secreted protein by using FISH to identify the cells that produced the protein and IHC to identify where the secreted protein is located.
- Detect transplant cells: ImmunoFISH can be used to detect transplant cells with various cellular markers.
- Disset the regulation of gene expression: ImmunoFISH can be used to dissect the regulation of gene expression by using ISH and IHC to differentiate between gene regulation at the transcriptional level and at the translational or posttranslational level.
Features:
- High accuracy and sensitively
- Fast turnaround time
- Competitive pricing
Creative Bioarray offers ImmunoFISH Analysis for your scientific research as follows:
- Probe design
- Probe synthesis
- Optimization of FISH/IHC protocols
- Perform ISH first followed by IHC
- Imaging
- Data analysis
Quotation and ordering
Our customer service representatives are available 24hr a day! We thank you for choosing Creative Bioarray at your preferred ImmunoFISH Analysis Service.
References
- Calattini S, et al. Detection of EBV genomes in plasmablasts/plasma cells and non-B cells in the blood of most patients with EBV lymphoproliferative disorders using Immuno-FISH[J]. Blood, 2010: blood-2010-05-285452.
- Duval C, et al. ImmunoFISH is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas[J]. PloS one, 2014, 9(6): e100342.
- Sempere L F, et al. Fluorescence-based codetection with protein markers reveals distinct cellular compartments for altered MicroRNA expression in solid tumors[J]. Clinical cancer research, 2010: 1078-0432. CCR-10-1152.
- Martinez-Ramirez A, et al. (2004) Simultaneous detection of the immunophenotypic markers and genetic aberrations on routinely processed paraffin sections of lymphoma samples by means of the FICTION technique. Leukemia 18: 348–353.
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