Passive Cutaneous Anaphylaxis Model
Passive Cutaneous Anaphylaxis (PCA) is an immediate dermal inflammation mediated by the combination of specific antigen and exogenous IgE antibodies on the surface of mast cells. The mice/rats were injected intradermally with antiserum followed by a latent period. Then they were intravenous (IV) injected with Evans blue dye bound to the antigens, which triggered the release of histamine, protease and inflammatory factors that lead to allergic and inflammatory reactions.
Creative Bioarray, with a prestigious team in the industry, focuses on providing customized experiment services to help customers assess the efficacy of compounds and study the associated pathological mechanisms by passive cutaneous anaphylaxis model.
Species available
- mouse PCA
- rat PCA
Our capabilities
- We test the anti-inflammatory effect of novel compounds.
- We detect the dye extracted from per ear of mice/rats using spectrophotometer.
- We evaluate various cytokines through ELISA, etc.
Assays available
- Absorbancy of Evans Blue
- Histopathological analysis
- Cytokine analysis
- PK/PD blood collections
With extensive experience in the field of PCA, we are confident to help you to overcome any upcoming challenges. Our experts are fully capable of customizing our protocols and assays to meet your specific needs. With our help, we wish to facilitate your research with high efficiency.
Study examples
Figure. 1. Spinacetin inhibits IgE-mediated PCA reaction. (A) The mice were injected intradermally with 80 ng of anti-DNP IgE into one ear. After 24 h, the mice were intravenously injected with 60 mg of DNP-HSA containing 1% Evans blue. Spinacetin was orally administered 1 h before Ag administration. (B,C) The dye extracted from per ear was detected using spectrophotometer.
Quotation and ordering
If you have any special needs or questions regarding our services, please feel free to contact us. We look forward to cooperating with you in the future.
Reference
Ning J, et al. Spinacetin Suppresses the Mast Cell Activation and Passive Cutaneous Anaphylaxis in Mouse Model[J]. Frontiers in Pharmacology, 2018, 9:824-.
