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Migration is a key property of live cells and critical for normal development, immune response, and disease processes such as cancer metastasis and inflammation. Methods to examine cell migration are very useful and important for a wide range of biomedical research such as cancer biology, immunology, vascular biology, cell biology and developmental biology. With comprehensive list of cell lines in our inventory, Creative Bioarray provides cell migration and Invasion Assay Services to all of our customers.
Creative Bioarray Cell Migration and Invasion Assay advantages
Technique and procedures
Two main types of methods are available at Creative Bioarray:
Cell culture wound-healing assays
The wound-healing assay is simple, inexpensive method to study directional cell migration in vitro. This method mimics cell migration during wound healing in vivo. A “wound” is generated on a confluent cell monolayer. The speed of wound closure and cell migration can be quantified by taking snapshot pictures with a regular inverted microscope at several time intervals. More detailed cell migratory behavior can be documented using the time-lapse microscopy system.
Suitable Applications: Measure differences in migration rates between treated and untreated cells.
Wound-healing assays based on (A) scratching, (B) stamping, (C) solid barrier, (D) liquid barrier, (E) droplet chemical assay, (F) microfluidic chemical assay, (G) electrical assay, and (H) laser ablation.
Transwell cell migration and invasion assay (Boyden chamber assay)
Transwell migration assays have been widely used for studying the motility of different types of cells including metastatic cancer cells. By using this method, we measure the capacity of cell motility and invasiveness toward a chemo-attractant gradient.
Suitable Applications: Measure effect of chemoattractant on migration rates
Other migration assays available
Other migration assays
Figure1: Results for wound-healing assays
Figure 2: Transwell migration and invasion assays of T24 and 5637 bladder urothelial cancer cells.
Quotations and ordering
Call us today at 631-626-9181, or email us at firstname.lastname@example.org for a quotation or if any questions! Our customer service representatives are available 24hr a day!
Cell Line Testing and Assays: