Papain-Induced Osteoarthritis (OA) Model

If you're looking for a reliable and experienced partner to evaluate the efficacy of your drug candidates on osteoarthritis (OA), Creative Bioarray is the perfect choice for you. We have been working in this field for years, and have established an OA model induced by papain which is widely accepted in the industry. Our team of experts, coupled with a set of analytical tests, ensures that we provide high-quality and reliable data to our clients. With our comprehensive experience and expertise, you can trust us to help you achieve your research goals insert most efficiently and accurately possible.

OA is the most common type of arthritis affecting preferentially the hands, knees, hips, lower back, and neck. It occurs when the smooth cushion between bones, called cartilage, becomes damaged after a sustained inflammatory response and thus joints become painful, swollen, and hard to move. Papain is a protease that can degrade the extracellular matrix and contribute to OA. The rat model with papain injection changed gait and pain responses and cartilage scoring.

Our Papain-Induced Osteoarthritis (OA) Model

• Available Animal
  Rat
• Modeling Method
  After anesthetization, rats are given the intra-articular injection of papain.
• Endpoints
  • Behavioral test
  • Histology analysis (knee joint): H&E staining, Safranin-O/Fast Green staining
  • Mankin score
  • Serum analysis: cytokine, SOD, MDA, etc.
  • Body weight
  • Other customized endpoints

Example Data

Fig. 1 Effect of non-weight-bearing exercise on knee joint pathology in papain-induced rats with KOA. (A) Pathological features of the knee joint of rats with KOA observed after H&E staining. (B) Mankin score of cartilage tissue of knee joint in rats with KOA

Fig. 2 Effect of non-weight-bearing exercise on inflammatory factors in the knee joint of papain-induced rats with KOA. Detection of the expression of inflammatory factors IL-1β, IL-6, and TNF-α in the serum of rats with KOA using ELISA.

In addition, we also provide other OA models that maybe you are interested in:

• Monosodium Iodoacetate (MIA)-Induced Osteoarthritis (OA) Model
• Medial Meniscal Tear (MMT)-Induced Osteoarthritis (OA) Model
• Anterior Cruciate Ligament Transection (ACLT)-Induced Osteoarthritis (OA) Model

Quotation and Ordering

Creative Bioarray has established a diverse range of disease models tailored to the specific needs of our clients. These models serve as powerful tools for researchers to delve deeply into the mechanisms underlying various diseases and assess the potential of drug candidates. If you are interested in our services, please feel free to contact us at any time or submit an inquiry to us directly.

References

1. Wang, K., et al. Non-weight-bearing exercise attenuates papain-induced knee osteoarthritis in rats via the TLR4/MyD88/NF-κB signaling pathway. Journal of Orthopaedic Surgery and Research, 2023, 18(1): 695.
2. Kuyinu, E.L., et al. Animal models of osteoarthritis: classification, update, and measurement of outcomes. Journal of orthopaedic surgery and research, 2016, 11: 1-27.
3. Chapman, J.H., et al. Animal Models of Osteoarthritis: Updated Models and Outcome Measures 2016–2023. Regenerative Engineering and Translational Medicine, 2023: 1-20.