Prostate Tumor Cells
Prostate cancer is one of the most prevalent malignancies in men, with a complex progression from androgen-dependent to castration-resistant disease. Our collection of prostate tumor cell lines provides essential tools for studying tumor biology, therapeutic resistance, and the molecular mechanisms underlying disease progression.
This portfolio includes classic and well-characterized models, from the foundational LNCaP to more aggressive lines like PC-3, enabling research into hormone response, metastasis, and novel treatment strategies.
Classic Models AR-Profiled Metastatic Validated
Key Features & Expertise
Our prostate tumor cell lines are selected and characterized to address critical research challenges
Comprehensive Model Spectrum
- Coverage of disease progression: from androgen-sensitive (LNCaP) to castration-resistant (PC-3, DU 145) models
- Representation of common metastatic sites, such as bone (PC-3)
- Includes models with key genetic alterations (TP53, PTEN, AR variants) relevant to human disease
Androgen Receptor (AR) & Pathway Characterization
- Detailed AR status: expression level, mutation, and splice variant information
- Profiling of associated pathways: PI3K/AKT, MAPK, and DNA repair
Authenticated for Reliable Research
- STR-authenticated to ensure genetic identity and prevent cross-contamination
- Routinely tested and certified mycoplasma-free
- Supplied with consistent growth performance and comprehensive culture support
FAQ
What are the main differences between LNCaP, PC-3, and DU 145 cell lines?
These are three of the most widely used prostate cancer models, each representing a distinct disease stage. LNCaP is androgen-sensitive, expresses the androgen receptor (AR), and is derived from a lymph node metastasis. PC-3 and DU 145 are both androgen-independent, castration-resistant lines. PC-3 is highly metastatic and derived from a bone metastasis, while DU 145 comes from a brain metastasis and is often used to study advanced, treatment-resistant disease.
How do I choose a cell line for studying androgen receptor signaling?
For AR-focused studies, LNCaP and its derivatives (like C4-2, which models progression to castration resistance) are primary choices as they express functional AR. For studying AR-negative or androgen-independent pathways, PC-3 or DU 145 are more appropriate. The key is matching the model's AR status (wild-type, mutant, or null) to your specific research question on hormone signaling or resistance.
Are there cell lines suitable for 3D culture or organoid models?
Yes, many prostate cancer cell lines, particularly LNCaP and its more advanced derivatives, can be cultured in 3D to form spheroids or used to generate organoids. These models are valuable for studying tumor morphology, drug penetration, and mimicking the in vivo microenvironment more accurately than 2D monolayers.
How are the cell lines authenticated and quality-controlled?
All cell lines undergo STR (Short Tandem Repeat) profiling to confirm their unique genetic identity and rule out cross-contamination. They are also routinely screened and certified to be free of mycoplasma and other common contaminants to ensure experimental integrity and reproducibility.
What is the recommended culture medium and conditions?
Standard protocols are provided with each cell line. Most prostate cancer lines are cultured in RPMI-1640 or DMEM supplemented with 10% FBS. For androgen-sensitive lines like LNCaP, charcoal-stripped serum is often used in experiments to study androgen regulation. Specific recommendations are included in the product documentation.