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Photo-aging & UV-Protection

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Photo-aging & UV-Protection

Photo-aging & UV-Protection

Exposure to solar UV radiation (UVA, thermal component, UVB) is the main environmental factor that causes premature aging of the skin (photo-aging). Human skin aging resulting from UV irradiation is a cumulative process that occurs based on the degree of sun exposure and the level of skin pigment. UV irradiation induces matrix metalloproteinases (MMPs) responsible for alterations in the collagenous extracellular matrix of connective tissue, resulting in impaired integrity. On a molecular level, UV radiation from the sun attacks keratinocytes and fibroblasts, resulting in the activation of cell surface receptors, which initiate signal transduction cascades. This in turn leads to a variety of molecular changes, which causes a breakdown of collagen in the extracellular matrix and a shutdown of new collagen synthesis.

In order to identify most efficient applications of the new ingredients of UV-Protection, the need for in vitro testing in cosmetic or dermatological industries is greatly growing nowadays, because of below aspects: firstly, regulatory affairs that include consumer safety and strict ban of animal use in testing. Secondly, there is a big advantage regarding research and development because of faster screening and access to more informative tests. Finally, this leads to on the evidence based communication with consumers.

Your Needs

  • You are looking for a skin model to perform in vitro photo-aging or UV protection testing?
  • You wish to screen ingredients, and characterize the efficacy or toxicity or safety of any products or chemicals of UV-protection?
  • You'd like to find a customized in vitro testing service of photo-aging or UV-protection?

Our Capability

Creative Bioarray provides in vitro 3D skin tissue models and evaluation assays performed for cosmetic or dermatological efficacy and claims support of photo-aging & UV protection.

In vitro 3D skin models available

Assays available

  • Phototoxicity Assay (OECD Test Guideline 432)
  • Evaluating effects of different formulations, ingredients by using 3D skin models
  • Other customized assays by using 3D skin models

Endpoints

  • Inflammatory mediator release (IL-8, PGE2, IL-6, TNF-alpha, etc.)
  • Free radical production
  • DNA damages (CPD, sunburn cells, comet assay, etc.)
  • Stress response (p53, HSPs, NRF-2 pathway)
  • Metalloproteinase expression or production
  • Antimicrobial peptide production (beta-defensins, S100A7, etc.)
  • Apoptosis (caspase-3, sunburn cells)
  • Cell viability or metabolism

Techniques

  • qPCR, qPCRarray, RT-PCR
  • Epidermal separation
  • Immunofluorescence
  • ELISA
  • RNA extraction
  • Protein extraction
  • Macroscopy

Assay Examples

Photo-aging & UV-Protection Fig 1. Cosmetic ingredient inhibits UVA-induced MMP-1 release

Photo-aging & UV-Protection Fig 2. Comparison of relative expression of MMP-1 and MMP-3 in UVB group and control group.

Related Products and Services

Choose our models to perform screening assays in house, or choose our assays and services directly!!

Call us today at 631-626-9181, or email us at for a technical supports if any questions! Our customer service representatives are available 24hr a day! We thank you for choosing Creative Bioarray services!

In vitro Skin Models:


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CONTACT US USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: 

Europe
Tel: 44-207-097-1828
Email: