Our Promise to You

Guaranteed product quality, expert customer support

Our Promise to You

T2

  • Specification
  • Recommended Products
Cat.No.
CSC-C6231X
Description
Established by PEG-mediated fusion of the B-lymphoblastoid cell line (B-LCL) LCL 721.174 with an 8-azaguanine and ouabain-resistant variant of the T-LCL CEM (CEMR.3); cells were described to synthesize HLA-A2 and -B5 RNA, but express only small amounts of A2 and no B5 antigen on the cell surface; subclone of the T1 cell line which has lost both CEMR-derived copies of chromosome 6; cell line also known as 174xCEM.T2
Species
Human
Source
somatic cell hybrid
Recommended Medium
Culture Properties
suspension
Morphology
round to polymorphic cells growing in suspension singly and in clumps
Karyotype
Human flat-moded hypertriploid karyotype with 5% polyploidy; 69-78<3n>XX, +2, +11, +13, +14, +16, +20, +20, +21, +mar, add(X)(q11), del(7)(q31.3), add(8)(p11), t(8;9)(p11;p24), del(9)(q21)x1-2, add(14)(q32)x1-2, add(16)(q11), add(18)(q23); derived from CC
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD2 -, CD3 -, CD4 +, CD5 -, CD6 -, CD7 +, CD8 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 -, CD138 -, TCRalpha/beta -, TCRgamma/delta -, HLA-DR -
Viruses: PCR: EBV +, HBV
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
  • Customer Review
Q:
How can we ensure the integrity of tumor cell lines during quality control processes?
A:

Integrity can be ensured by regularly authenticating cell lines, testing for mycoplasma contamination, and monitoring genetic stability.

Ask a Question

Average Rating: 5.0    |    1 Scientist has reviewed this product

Highly viable

The cells were not only pure but also highly viable, ensuring accurate and reliable experimental results. The supplier provides detailed protocols and documentation, allowing for seamless integration into my research workflow. Would reorder this product.

12 May 2023


Ease of use

After sales services

Value for money


Write your own review

Customer Support & Price Inquiry

Verification Code