T2

Cat.No.: CSC-C6231X

Species: Human

Source: somatic cell hybrid

Morphology: round to polymorphic cells growing in suspension singly and in clumps

Culture Properties: suspension

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Cat.No.

CSC-C6231X

Description

Established by PEG-mediated fusion of the B-lymphoblastoid cell line (B-LCL) LCL 721.174 with an 8-azaguanine and ouabain-resistant variant of the T-LCL CEM (CEMR.3); cells were described to synthesize HLA-A2 and -B5 RNA, but express only small amounts of A2 and no B5 antigen on the cell surface; subclone of the T1 cell line which has lost both CEMR-derived copies of chromosome 6; cell line also known as 174xCEM.T2

Species

Human

Source

somatic cell hybrid

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

round to polymorphic cells growing in suspension singly and in clumps

Karyotype

Human flat-moded hypertriploid karyotype with 5% polyploidy; 69-78<3n>XX, +2, +11, +13, +14, +16, +20, +20, +21, +mar, add(X)(q11), del(7)(q31.3), add(8)(p11), t(8;9)(p11;p24), del(9)(q21)x1-2, add(14)(q32)x1-2, add(16)(q11), add(18)(q23); derived from CC

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD2 -, CD3 -, CD4 +, CD5 -, CD6 -, CD7 +, CD8 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 -, CD138 -, TCRalpha/beta -, TCRgamma/delta -, HLA-DR -
Viruses: PCR: EBV +, HBV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How can we ensure the integrity of tumor cell lines during quality control processes?

Integrity can be ensured by regularly authenticating cell lines, testing for mycoplasma contamination, and monitoring genetic stability.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Highly viable

The cells were not only pure but also highly viable, ensuring accurate and reliable experimental results. The supplier provides detailed protocols and documentation, allowing for seamless integration into my research workflow. Would reorder this product.

12 May 2023

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For research use only. Not for any other purpose.