Epigenetic Induction of Cell Growth Service (Non-Viral Cell Immortalization Method)

Creative Bioarray is offering epigenetic induction of cell growth service for the cell immortalization. With the Epigenetic Approach, we have established Immortalized Chicken Hepatocytes, Immortalized Monkey Hepatocytes (Cat No.: CSC-I2072Z), Immortalized Dog Hepatocytes and Immortalized Human T Cells (Cat No.: CSC-I2269Z), which their primary cells are very difficult to immortalize in traditional ways. From this experience, Creative Bioarray has much confidence in establishing other Immortalized immune cells (e.g. nature killer cells, Dendritic Cells, CD4+ CD8+ T Cells).

The Epigenetic Induction of Cells Growth is a great method to establish immortalized cell lines that are hard to immortalize in other methods:

Key Features

Work Flow

Case Studies

In 2023, we established Immortalized Human T Cells using Epigenetic Approach successfully. Immortalized human T cells from Creative Bioarray can be cultured continuously for 20 passages.

Figure 1. Immortalizing human T cells by Epigenetic Induction of Cell Growth. Growing T cells at Day 2 after stimulation. NOTE: Non fused control T cells can be cultured in the same conditions for 2 weeks before showing deteriorating cell growth.

Figure 2. Immortalizing human T cells by Epigenetic Induction of Cell Growth. Growing T cells at week 3 after stimulation.

We established Immortalized Monkey Hepatocytes using Epigenetic Approach. Immortalized Monkey Hepatocytes from Creative Bioarray can be cultured continuously for 20 passages.

Figure 3. Morphology of Immortalized Cynomolgus Monkey Hepatocytes

At first, we began using Lentiviral transfection of hTert-P53 siRNA under the control of Dox (Tet-on system) to generate Immortalized Chicken Hepatocytes as the client requested. Unfortunately, after 3 weeks of selection culture, no cells were able to survive the selection process. Then we utilized Epigenetic induction of Cell Growth, and the immortalized chicken hepatocytes were successfully passaged 20 times.

Figure 4. Morphology of Immortalized Chicken Hepatocytes

Quotation and Ordering

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Explore Other Options

• In Vitro DMPK Services
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• Cell Immortalization Service
• Custom Host Cell Line Development
• Immortalized Cell Line Engineering Service
• Immortalizing Cells for Cell-Cultured Meat
• T Cell Immortalization Service

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