Skin sensitization is defined as allergic response to a substance after skin contact. A skin sensitiser refers to a substance that will lead to an allergic response following skin contact as defined by the United Nations Globally Harmonized System of Classification and Labelling of Chemicals.
The assessment of skin sensitization has typically involved the use of laboratory animals. The classical method is using guinea-pigs. While, more recently mechanistically-based in chemico (OECD TG 442C) and in vitro (OECD TG 442D) test methods have been adopted for contributing to the evaluation of the skin sensitization hazard potential of chemicals as a valid animal test alternative.
Fig 1. OECD flow diagram of the pathways associated with skin sensitization. The Adverse Outcome Pathway (AOP) framework provides a structure for collecting, organizing and evaluating the available data that describe the compound and the events resulting in an adverse outcome at a biological level of organization.
Creative Bioarray team provides in vitro skin models and in vitro skin sensitization assays in order to support pharmaceutical and cosmetic companies for their drug or product development related to skin sensitization.
- You are looking for an in vitro skin model to evaluate a skin sensitiser?
- You wish to evaluate chemicals, ingredients or formulations of drugs or products for their skin sensitization hazard?
- You'd like to find a customized in vitro testing service related to skin sensitization?
In vitro skin models available
Creative Bioarray can provide a range of services to evaluate skin sensitization by using our skin models or cell lines:
- The In Vitro Sensitization Assay (IVSA) is a keratinocyte activation test. IVSA uses an ELISA method to measure IL-18 release from a topically treated reconstructed 3D human keratinocyte tissue model.
- The Human Cell Line Activation Test (h-CLAT) is a dendritic cell activation test. The h-CLAT uses flow cytometry to measure CD86 and CD54 expression on treated THP-1 Cell line in culture, based on OECD TG Draft (May 2015).
- The Direct Peptide Reactivity Assay (DPRA) is an in chemico method used to predict epidermal protein binding. Binding of epidermal proteins is the molecular initiating event on the AOP. The DPRA uses HPLC to measure the depletion of synthetic peptides in solution following exposure to test chemicals, based on OECD TG 442C (February 2015).
- The KeratinoSens Assay is a cell-based reporter gene assay which identifies skin sensitizers by measuring the induction of luciferase under the control of the antioxidant response element (ARE) derived from the human AKR1C2 gene. In the proposed Adverse Outcome Pathway (AOP) leading to skin sensitization, this method addresses the second key event, gene expression in keratinocytes associated with the antioxidant/electrophile response element (ARE)-dependent pathway. The assay was endorsed by ECVAM, and the OECD followed with a test guideline (OECD 442D).
Fig 2. Gene expression analysis after treatment for 24 hours with sensitizers
Related Products and Services
Choose our models to perform screening assays in house, or choose our assays and services directly!!
- Full thickness skin model (FTSK)
- Reconstructed human epidermis model (RHE)
- THP-1 Cell line
- Skin sensitization test services
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In vitro Skin Models:
For research use only. Not for any other purpose.