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The MT-2 cell line was derived from normal human cord leukocytes of a healthy donor by co-cultivation with leukemic cells from an adult T-cell leukemia (ATL) patient. This unique cell line offers a valuable model for studying certain aspects of T-cell biology and their therapeutic potential.
One significant characteristic of the MT-2 cell line is its ability to produce TGF-β, an immunosuppressive cytokine, which may contribute to the cell line's suppressive activity. Additionally, the MT-2 cell line has been shown to have the phenotypic and functional characteristics of human regulatory T cells (Tregs), suggesting that it may be used as a human Treg-like cell line for studies related to these cells. In addition to its potential role in Treg studies, the MT-2 cell line is also used to detect syncytium-inducing (SI) variants of HIV. In the MT-2 cell culture assay, MT-2 cells are cultivated with cell-free supernatants from HIV-infected PBMC cultures. The inoculated MT-2 cell cultures are then monitored for the development of a typical cytopathic effect, providing valuable insights into the behavior of HIV.
Melphalan Improves Toxicity of Arsenic Trioxide in Adult T-cell Leukemia/Lymphoma Cells
Adult T-cell leukemia/lymphoma (ATLL) is a hematologic neoplasm with poor prognosis. Melphalan is an alkylating anti-cancer agent, and arsenic trioxide (ATO) is routine chemotherapy drug for ATLL with low response rate. Due to the significant challenge that chemoresistance poses in treating ATLL, we aimed to investigate the potential of melphalan to enhance the effects of ATO as a combinatorial treatment approach for ATLL.
MT-2 cells were exposed to different concentrations of melphalan and ATO and viability was evaluated by alamarBlue assay. Upon IC50 determination, cells were treated with 0.5 μg/ml melphalan and 2 μM ATO for 72 h, and changes induced on the cell cycle were analyzed by PI staining and flow cytometry, while the expression of candidate genes was assessed by quantitative PCR.
Fig. 1. Viability of MT-2 cells after 72 h treatment with melphalan and ATO, alone and in combination (A). Flow cytometry histograms (B) and quantitative analysis (C) of the cell cycle upon single and combinatorial use of melphalan and ATO (Khodadadi, Faeze, et al. 2025).
RELA/C Knockout Reduced FOXP3 Expression and Immune-suppressive Function of Tregs.
Mutations in NF-κB-related molecules result in combined immunodeficiency characterized by recurrent infection. This study aimed to investigate the association between mutations in NF-κB family members (RELA, RELB, and RELC) and regulatory T cell (Treg) function in humans. Similar to that observed in knockout mice, RELA or RELC knockout in MT-2 cells and freshly isolated Tregs reduced FOXP3 expression and the immune suppressive function of Tregs. Additionally, PD-L1 expression in effector T cells and Tregs decreased considerably following RELC knockdown. These findings demonstrated that the deletion of RELA or RELC resulted in the loss of Treg-like phenotype and function owing to the downregulation of FOXP3 expression.
Fig. 2. FOXP3 expression was partially lost in MT-2 cells bearing RELA and RELC knockout (Sato, Yohei, Yamato Hanawa, and Akihito Tsubota. 2024).
Plasma cells are white blood cells that produce disease- and infection-fighting antibodies in your body. Myeloma cells prevent the normal production of antibodies, leaving your body's immune system weakened and susceptible to infection.
MT-2 cells have the ability to produce TGF-β, an immunosuppressive cytokine, and they have been shown to have the phenotypic and functional characteristics of human regulatory T cells (Tregs).
Given their phenotypic and functional characteristics of human Tregs, MT-2 cells may be used as a human Treg-like cell line for studies related to these cells.
MT-2 cells, with their unique characteristics and origins, serve as a valuable tool in the study of T-cell biology, Tregs, and HIV.
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Average Rating: 4.7 | 3 Scientist has reviewed this product
Well packaged
Once I received the cells, the product was well packaged and labeled, making it easy to use.
23 Jan 2023
Ease of use
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Good performance
The MT-2 cells have performed well in our experiments, allowing us to make significant progress in our studies.
25 Jan 2024
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Prompt responses
The team at Creative Bioarray was also very helpful, providing prompt and informative responses to our queries.
02 Dec 2023
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