Established by irradiation of the adherent cells in long-term bone marrow cultures derived from C3H/HeNSlc strain mice
Murine hypertriploid karyotype
adherent fibroblastic cells growing in monolayers
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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Defining human dendritic cell progenitors by multiparametric flow cytometry
Authors: Breton G, Lee J, Liu K, et al.
The Nature protocols. 2015, 10(9): 1407-1422.
Sinusoidal ephrin receptor EPHB4 controls hematopoietic progenitor cell mobilization from bone marrow
Authors: Kwak H, Salvucci O, Weigert R, et al.
The The Journal of clinical investigation, 2016, 126(12): 4554.