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Species: Human

Source: gastric adenocarcinoma

Culture Properties: monolayer

Morphology: both spindle-shaped or oval cells growing in monolayers and single round cells or clumps in suspension

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Established from the poorly differentiated adenocarcinoma of the stomach (medullary type) of a 62-year-old woman
gastric adenocarcinoma
Recommended Medium
Culture Properties
both spindle-shaped or oval cells growing in monolayers and single round cells or clumps in suspension
Human hypertriploid karyotype with 8% polyploidy - 70-73<3n>XX/XXX, -X, -2, -2, -4, -5, -5, +6, -11, -13, -13, -14, -18, -19, -20, -21, -22, +20mar, add(X)(q1?), der(1)(1pter->p21::1p11->q32::1q44->q11::?), i(2p), der(3)t(3;?9)(p14;q13/21), del(4)(q21), d
Quality Control
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
How to remove fibroblasts by enzymatic digestion in the process of purifying tumor cells?

Fibroblasts are more sensitive to enzymes, so they can be removed more easily than tumor cells when digested with trypsin or collagenase. The digestion can be observed under the microscope and terminated immediately after the fibroblasts are shed, and the treatment can be repeated several times to obtain purer tumor cells.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Detailed instruction

Each product comes with a detailed instruction manual that includes the protocol for that cell culture, media composition, etc., which is very friendly for novice experimenters.

23 Apr 2022

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