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Multicolor FISH (M-FISH) assays are used for a precise assessment of complex chromosomal rearrangements. This technique uses all whole-chromosome painting probes in multiplex-FISH and spectral karyotyping. Thus, marker chromosomes, complex chromosomal rearrangements, and all numerical aberrations can be visualized simultaneously in a single hybridization experiment. M-FISH is a kind of versatile tool for precise chromosome separation and for rapid chromosome classification help speeding up the workflow. Apart from that, the analysis of M-FISH images is easy even if aberrations are involved.
M-FISH complements the standard cytogenetic methods, which is helpful in deciphering complex chromosomal rearrangements and is used to identify non-random structural chromosome rearrangements not detectable by other methods. Genomic alteration of many tumor cell lines was characterized by M-FISH.
Fig1. Representative M-FISH karyotype of K562 cell.
Kamel, Yasser Mostafa, et al. Fluorescence in situ hybridization assays designed for del (7q)
detection uncover more complex rearrangements in myeloid leukaemia cell lines. (2014).
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