Multicolor FISH (M-FISH) Analysis

Multicolor FISH (M-FISH) assays are used for a precise assessment of complex chromosomal rearrangements. This technique uses all whole-chromosome painting probes in multiplex-FISH and spectral karyotyping. Thus, marker chromosomes, complex chromosomal rearrangements, and all numerical aberrations can be visualized simultaneously in a single hybridization experiment. M-FISH is a kind of versatile tool for precise chromosome separation and for rapid chromosome classification help speeding up the workflow. Apart from that, the analysis of M-FISH images is easy even if aberrations are involved.

M-FISH complements the standard cytogenetic methods, which is helpful in deciphering complex chromosomal rearrangements and is used to identify non-random structural chromosome rearrangements not detectable by other methods. Genomic alteration of many tumor cell lines was characterized by M-FISH.

Fig1. Representative M-FISH karyotype of K562 cell.
Kamel, Yasser Mostafa, et al. Fluorescence in situ hybridization assays designed for del (7q)
detection uncover more complex rearrangements in myeloid leukaemia cell lines. (2014).

Our M-FISH has unparalleled advantages:

• Rapid analysis of metaphase spread
• Ability to analyze complex cases with multiple chromosomal rearrangements, identification of marker chromosomes
• Ability to distinguish different imbalanced situations detectable in aCGH
• Ability to accurately karyotype single metaphases without selection

Features:

• High accuracy and sensitively
• Fast turnaround time
• Competitive pricing

References

1. Kamel, Yasser Mostafa, et al. "Fluorescence in situ hybridization assays designed for del (7q) detection uncover more complex rearrangements in myeloid leukaemia cell lines. (2014).
2. Liehr, Thomas, et al. "Multicolor FISH methods in current clinical diagnostics." Expert review of molecular diagnostics13.3 (2013): 251-255.
3. Kearney, L. "Multiplex-FISH (M-FISH): technique, developments and applications." Cytogenetic and genome research 114.3-4 (2006): 189-198.

Explore Other Options

• In Situ Detection of CAR-T Cells & Oncolytic Viruses
• In Situ Detection of Chemokines/Cytokines
• In Situ Detection of Virus
• CARD-FISH for Environmental Microorganisms
• CAR-T/CAR-NK Target Assessment Service (ISH)

• In Situ Hybridization (ISH) & RNAscope Service
• Animal Chromosome Analysis (G-banded) Service
• Digital ISH Image Quantification and Statistical Analysis
• Droplet Digital PCR (ddPCR) Service
• FISH Probe Design, Synthesis and Testing Service

For research use only. Not for any other purpose.