Tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.
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Creative Bioarray provides Cell Cycle Analysis Service for all of our customers.
The cell cycle is the most fundamental and important process by which eukaryotic cells duplicate and divide. The cell cycle consists of two specific and distinct phases: interphase, consisting of G1 (Gap 1), S (synthesis), and G2 (Gap 2), and the mitotic phase; M (mitosis).
The technique Creative Bioarray uses is flow cytometry measuring cellular DNA content.
Cell cycle refers to the set of events through which a cell grows, replicates its genome, and ultimately divides into two daughter cells through the process of mitosis. Because the amount of DNA in a cell shows characteristic changes throughout the cycle, techniques known as cell cycle analysis can be used to separate a population of cells according to the different phases of cell cycle they are in, based on their varying DNA content.
By using flow cytometry, staining dyes are needed. Creative Bioarray can choose different dyes to perform the assays, including propidium iodide (PI), BrdU, 7-amino actinomycin-D (7-AAD), Hoechst 33342 and 33258, and 4’6’-diamidino-2-phenylindole (DAPI), based on the customer’s applications or requirements.
Figure. FACS output of cell cycle analysis based on varying DNA content
Quotations and ordering
If you want to perform the analysis in house, Creative Bioarray also provides Cell Cycle Analysis Kit to assist with your research.
Cell Line Testing and Assays: