ML-2

Cat.No.: CSC-C0200

Species: Homo sapiens (Human)

Source: Blood; Peripheral Blood

Morphology: round, single cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0200

Description

Established from the peripheral blood of a 26-year-old man with acute myeloid leukemia (AML M4) at diagnosis of AML (following T-non-Hodgkin lymphoma and T-ALL) in 1978; carries t(6;11)(q27;q23) leading to MLL-MLLT4 (MLL-AF6) fusion gene; sister cell lines are ML-1 and ML-3

Species

Homo sapiens (Human)

Source

Blood; Peripheral Blood

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS

Culture Properties

suspension

Morphology

round, single cells in suspension

Karyotype

Human near tetraploid karyotype - 92(84-94)<4n>XX, -Y, -Y, -7, -9, -10, -10, +11, +12, +12, +13, +13, -15, -16, -17, -17, +18, +18, -20, -20, +4mar, der(1)t(1;?)(p21;?)x2, del(6)(q23)x2, der(6)t(6;11)(q27;?q23)x2, ?der(11)t(6;11)(q27;?q23)/del(11)(q23)x2,

Disease

Acute Myeloid Leukemia

Quality Control

Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
mmunology: CD3 -, CD4 -, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +
Viruses: ELISA: rever

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

ML2

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

ML-2 cells are a well-known and extensively studied cell line that was established from the peripheral blood of a 26-year-old man in 1978. The blood sample was obtained at the time of diagnosis of acute myeloid leukemia (AML) of the M4 subtype. It is noteworthy that the patient had a medical history of T-cell non-Hodgkin lymphoma (T-NHL) and T-cell acute lymphoblastic leukemia (T-ALL) before the development of AML.

The establishment of the ML-2 cell line is significant because it carries a specific chromosomal translocation, t(6;11)(q27;q23), which results in the formation of the MLL-MLLT4 fusion gene, also known as the MLL-AF6 fusion gene. This fusion gene is a result of the rearrangement of the (MLL) gene on chromosome 11 and the myeloid/lymphoid or mixed-lineage leukemia translocated to the 4 (MLLT4) gene on chromosome 6.

ML-2 cells, along with their sister cell lines ML-1 and ML-3, have been instrumental in advancing our understanding of AML biology, particularly those associated with MLL gene rearrangements. Researchers have extensively utilized ML-2 cells to investigate the molecular mechanisms underlying leukemogenesis, disease progression, and response to various treatment modalities.

Lineage Infidelity of Human Myelogenous Leukemia Cell Lines

The organization and expression of the immunoglobulin heavy and light chain gene were analyzed in the human myeloblastic leukemic sublines, ML1, ML2, and ML3, and the human myeloid leukemic cell lines, HL-60, U937, THP1. One germline band of approximately 17 kb was detected in all the myeloid cell lines tested (Fig. 1). ML1, ML2, and ML3 cells showed an additional band of approximately 13.5 kb. Similarly, the joining (J_H) segment of the µ-gene, detected after hybridization of HindIII-digested DNA from the cell lines with a P-labeled J_H probe, resolved as a single 9-kb band in germline configuration in HL-60, U937, THP1, and K562 cells (Fig. 2A). However, the J_H segment was rearranged in ML1, ML2, and ML3 cells, as evidenced by a 9-kb germline band and a second band of 8 kb. Rearrangement of the J_H segment was also detected when the DNA samples were digested with EcoRI restriction endonuclease (Fig. 2B).

Fig. 1 C_µ-related sequences in DNA of human myeloid leukemic cell lines. (Lacrima K, et al., 2005)

Fig. 2 Configuration of J_H segment in human myeloid leukemic cell lines. (Lacrima K, et al., 2005)

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ML-2

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For research use only. Not for any other purpose.