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ML-2

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Cat.No.
CSC-C0200
Description
Established from the peripheral blood of a 26-year-old man with acute myeloid leukemia (AML M4) at diagnosis of AML (following T-non-Hodgkin lymphoma and T-ALL) in 1978; carries t(6;11)(q27;q23) leading to MLL-MLLT4 (MLL-AF6) fusion gene; sister cell lines are ML-1 and ML-3
Organism
Human
Karyotype
Human near tetraploid karyotype - 92(84-94)<4n>XX, -Y, -Y, -7, -9, -10, -10, +11, +12, +12, +13, +13, -15, -16, -17, -17, +18, +18, -20, -20, +4mar, der(1)t(1;?)(p21;?)x2, del(6)(q23)x2, der(6)t(6;11)(q27;?q23)x2, ?der(11)t(6;11)(q27;?q23)/del(11)(q23)x2,
Source
acute myelomonocytic leukemia
Culture Properties
suspension
Morphology
round, single cells in suspension
Recommended Medium
80-90% RPMI-1640 + 10-20% h.i. FBS
Quality Control
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
mmunology: CD3 -, CD4 -, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +
Viruses: ELISA: rever
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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