Oxazolone (4-ethoxylmethylene-2-phenyloxazol-5-one), a classical haptenating agent, is known to induce colitis in rats/mice that resemble human ulcerative colitis. Oxazolone induced colitis is characterized by hemorrhagic inflammation, augmented epithelial microulcerations and histopathological changes in the distal part of colon. In addition, this colitis is mediated by Th2 driven immune response. Creative Bioarray has many years of experience in the field of ulcerative colitis and provides pharmacodynamic services for customers to help them assess the efficacy of compounds and study the associated pathological mechanisms of ulcerative colitis through oxazolone induced coliti model.
- We utilize oxazolone induced colitis model to test the efficacy of drug candidates targeting IBD.
- We assess the severity of colitis through clinical analysis including weight loss, diarrhea and occult blood in stool.
- We evaluate various cytokines through IHC, ELISA, etc.
- Clinical analysis
- Histopathological evaluation
- Analysis of mRNA and protein levels
- Cytokine/chemokine analysis
With extensive experience in the field of IBD, we are confident to help you to overcome any upcoming challenges. Our experts are fully capable of customizing our protocols and assays to meet your specific needs. With our help, we wish to facilitate your research with high efficiency.
Figure. 1. Effects of PSB-0777 (0.4 mg/kg/day) alone or in combination with CSC (1 mg/kg/day) or dexamethasone (1 mg/kg/day) on body weight in rats with oxazolone-induced colitis. Each column represents the mean ± S.E.M (n = 6). *p < 0.05, significant difference versus control group; ap < 0.05, significant difference versus oxazolone group
Figure. 2. Hematoxylin and eosinstained sections of rat colon. Microscopic images refer to control rats (a) or oxazolone treated animals, either alone (b) or in the presence of PSB-0777 (0.4 mg/kg/day) (c) or dexamethasone (1 mg/kg/day) (d)
Figure. 3. Microscopic damage scores estimated for colon in rats under normal conditions or after oxazolone treatment, either alone or in the presence of PSB-0777 (0.4 mg/kg/day) alone or in combination with CSC (1 mg/kg/day), or dexamethasone (1 mg/kg/day) administration.
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Antonioli L, El-Tayeb A, et al. Anti-inflammatory effect of a novel locally acting A2A receptor agonist in a rat model of oxazolone-induced colitis[J]. Purinergic Signalling, 2017.
For research use only. Not for any other purpose.