Peritonitis Models
Peritonitis models is a widely used model for the induction of excessive inflammatory response characterized by leukocyte infiltration, synthesis of inflammatory mediators, etc. Two major peritonitis models are zymosan-induced peritonitis model and cecal ligation and puncture (CLP) model.
Zymosan, obtained from yeast cell walls, is a complex of polysaccharides. Zymosan-induced peritonitis are often considered as a simple and repeatable model that simulates acute inflammatory response with all known characteristics. In addition, acute inflammation induced by zymosan exposure peaks within a few hours and could be cleared afterward. Therefore, peritoneal wash fluid can be collected and analyzed within a short time frame. Creative Bioarray specializes in providing customized pharmacodynamic research services to help customers assess the efficacy of drug candidates and study the associated pathological mechanisms of peritonitis through peritonitis models.
Peritonitis models include but not limited to:
- Zymosan-induced peritonitis model
- Cecal ligation and puncture (CLP) model
Species available
- mouse
- rat
- rabbit
Our capabilities
- We test potential efficacy of novel anti-inflammatory compounds.
- We count the number of leukocytes using flow cytometry.
- We evaluate various cytokines through ELISA, etc.
Assays available
- Cytokine analysis in secrum and peritoneal wash fluid (PWF)
- PK/PD blood collections
With extensive experience in the field of peritonitis, we are confident to help you to overcome any upcoming challenges. Our experts are fully capable of customizing our protocols and assays to meet your specific needs. With our help, we wish to facilitate your research with high efficiency.
Study examples
Figure. 1. Time courses of leukocytes infiltrated into peritoneal cavity after an intraperitoneal administration of zymosan (1 or 10 mg). (A) Total cell number, (B) cell number of PMNs as determined by CD115-Gr-1+ cells, (C) cell number of macrophages as determined by CD115+Gr-1- cells, (D) cell number of eosinophils as determined by CCR3+ Siglec-F+ cells.
Figure. 2. Concentration time courses of IL-1b, TNF-a, IFN-c and MIP-1a in peritoneal wash fluid (PWF) after an intraperitoneal administration of zymosan or vehicle control in mice.
Quotation and ordering
If you have any special needs or questions regarding our services, please feel free to contact us. We look forward to cooperating with you in the future.
Reference
Fujieda Y, et al. Inflammation and Resolution Are Associated with Upregulation of Fatty Acid β-Oxidation in Zymosan-Induced Peritonitis[J]. PLOS ONE, 2013, 8.
