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HCT-116

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Cat.No.
CSC-C0586
Description
Established from the primary colon carcinoma of an adult man; cells were described to carry a RAS mutation in codon 13 and to be tumorgenic in nude mice
Organism
Human
Karyotype
Human near-diploid karyotype with 3% polyploidy - 45(44-46)<2n>X/XY, add(10)(q24-26), der(16)t(8;16)(q13;p13), der(18)t(17;18)(q13-21;p11.3) - resembles published karyotypes
Source
colon carcinoma
Culture Properties
monolayer
Morphology
epithelial-like small cells growing as monolayer
Recommended Medium
90% DMEM + 10% h.i. FBS
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Viruses: PCR:
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
FAT10, an ubiquitin-like protein, confers malignant properties in non-tumorigenic and tumorigenic cells Authors: Authors: Gao Y, Theng S S, Zhuo J, et al.
The The Carcinogenesis. 2013: bgt407
CSC-6317W
CSC-C0204
CSC-C0253
CSC-C0256
CSC-C0300
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