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HCT-116

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Cat.No.
CSC-C0586
Description
Established from the primary colon carcinoma of an adult man; cells were described to carry a RAS mutation in codon 13 and to be tumorgenic in nude mice
Organism
Human
Source
colon carcinoma
Recommended Medium
90% DMEM + 10% h.i. FBS
Culture Properties
monolayer
Morphology
epithelial-like small cells growing as monolayer
Karyotype
Human near-diploid karyotype with 3% polyploidy - 45(44-46)<2n>X/XY, add(10)(q24-26), der(16)t(8;16)(q13;p13), der(18)t(17;18)(q13-21;p11.3) - resembles published karyotypes
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Viruses: PCR:
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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