Established from the primary colon carcinoma of an adult man; cells were described to carry a RAS mutation in codon 13 and to be tumorgenic in nude mice
epithelial-like small cells growing as monolayer
Human near-diploid karyotype with 3% polyploidy - 45(44-46)<2n>X/XY, add(10)(q24-26), der(16)t(8;16)(q13;p13), der(18)t(17;18)(q13-21;p11.3) - resembles published karyotypes
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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