MIA PaCa-2

Cat.No.: CSC-C9492L

Species: Homo sapiens (Human)

Source: Pancreas

Morphology: Epithelial-like

Culture Properties: monolayer with floating cells

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Cat.No.

CSC-C9492L

Description

The MIA PaCa-2 cell line was established from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male in 1975 by A. Yunis, et al. These cells are described to express human colony stimulating factor, subclass I (CSF-I) and plasminogen activator. The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%. Sensitive to L-Asparaginase.

Species

Homo sapiens (Human)

Source

Pancreas

Recommended Medium

DMEM + 10% h.i.FBS

Culture Properties

monolayer with floating cells

Morphology

Epithelial-like

STR DNA Profile

Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 10,13
D5S818: 12,13
D7S820: 12,13
THO1: 9,10
TPOX: 9
vWA: 15

Karyotype

This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.

Application

The MIA PaCa-2 cell line can be used as tumourigenicity and enzymatic studies.

Disease

Pancreatic Ductal Adenocarcinoma

Quality Control

Tests for mycoplasma, bacteria and fungi were negative

Storage and Shipping

Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.