The MIA PaCa-2 cell line was established from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male in 1975 by A. Yunis, et al. These cells are described to express human colony stimulating factor, subclass I (CSF-I) and plasminogen activator. The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%. Sensitive to L-Asparaginase.
Homo sapiens (human)
monolayer with floating cells
STR DNA Profile
This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.
The MIA PaCa-2 cell line can be used as tumourigenicity and enzymatic studies.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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