Established from CV-1 Simian cells (cercopithecus aethiops) which were transformed by an origin-defective mutant of SV-40; cells are host cells for the propagation of pure populations of recombinant SV virus; classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS)
Hypotetraploid simian karyotype with 4% polyploidy - 102(95-108)<4n> - structural rearrangements present
fibroblast-like, adherent monolayers
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HCV -, HBV -, HIV -, HTLV-I/II -, SMRV -, SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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