CAL-27

Cat.No.: CSC-C0478

Species: Homo sapiens (human)

Morphology: Epithelial

Culture Properties: monolayer

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C0478

Description

The CAL-27 cells are established from the poorly differentiated squamous cell carcinoma of the tongue removed from a 56-year-old man before treatment in 1982. CAL 27 cells are epithelial, polygonal with a highly granular cytoplasm. Immunocytochemical studies show strong positive staining with anti keratin antibodies. The cells do not grow well in semi-solid medium. Marked inhibition of thymidine incorporation was observed in the presence of VP16 (etoposide), CCNU (1-[2-chloroethyl]-3-cyclohexyl-1-nitrosourea), VM26 (teniposide), ADM (adriamycin), CPA (cyclophosphamide), and MTX (methotrexate). CAL 27 cells were resistant to treatment with VDS (vindesine sulfate), CDP (cis-platinum) or ACTD (actinomycin D).

Species

Homo sapiens (human)

Tissue of Origin

Tongue

Recommended Medium

90% DMEM + 10% h.i.FBS

Culture Properties

monolayer

Morphology

Epithelial

STR DNA Profile

Amelogenin: X
CSF1PO: 10,12
D13S317: 10,11
D16S539: 11,12
D18S51: 13
D21S11: 28,29
D3S1358: 16
D5S818: 11,12
D7S820: 10
D8S1179: 13,15
FGA: 25
Penta D: 9,10
Penta E: 7
TH01: 6,9,3
TPOX: 8
vWA: 14,17

Karyotype

Human flat-moded hypodiploid karyotype with 3% polyploidy - 41(38-45)<2n>X, -Y, -4, +7, -10, -11, -18, -20, -21, -22, +2-3mar, add(3)(q21), i(3q), i(7p), del(8)(p21), i(9q), add(13)(p11), add(14)(p11) - sideline with additional der(3) and der(7) - resembl

Application

These cells have been used in studies of anti-tumour agents.

Disease

Squamous cell carcinoma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are non-cancerous growths in the mouth?

Most mouth growths are noncancerous. Warts, yeast infections, and repeated trauma (such as biting or rubbing against a sharp tooth edge) are common causes of noncancerous growths. Use of alcohol and tobacco and oral human papillomavirus (HPV) infection are risk factors for oral cancer.

Ask a Question

Average Rating: 5.0 | 1 Scientist has reviewed this product

Convenient

The convenience of tumor cells provided by Creative Bioarray ensures that our experiments are not hindered by limited availability.

17 June 2022

Ease of use
After sales services
Value for money

Write your own review

You May Also Need

CM-1010X

CS-001Y

KOSC-2 cl3-43

Description: Human tumor cells derived from oral cancer at stage III. The cells were originally cross contaminated with mouse cells but the mouse cells were eliminated in the JCRB cell bank and the cl3-43 ...

Cat#: CSC-C6819J INQUIRY

View Product

HN

Description: Established from the tumor of a 60-year-old Japanese man with moderately differentiated squamous cell carcinoma of the soft palate which was invasive into muscle layer; cell line was established from ...

Cat#: CSC-C0458 INQUIRY

View Product