Skin Tumor Cells
Skin cancers, including melanoma, squamous cell carcinoma, and other cutaneous malignancies, are among the most common cancers worldwide. Our collection of skin tumor and associated cell lines provides a diverse and well-characterized set of models to study the complex biology of skin cancers, from initiation and progression to metastasis and therapy resistance.
This portfolio encompasses aggressive melanoma lines like SK-MEL-2 and WM-266-4, squamous cell carcinoma models such as HSC-5, and valuable supporting cell types like dermal fibroblasts and microvascular endothelial cells. These tools enable comprehensive research into tumor-stroma interactions, angiogenesis, invasion, and the development of novel targeted and immunotherapeutic strategies.
Melanoma & SCC Models Stromal & Endothelial Cells Metastatic Potential Validated
Key Features & Expertise
Our skin tumor cell lines are characterized to support research across the spectrum of cutaneous oncology
Comprehensive Model Spectrum
- Melanoma models (e.g., SK-MEL-2, WM-266-4, MeCo 05) representing different stages and mutations
- Non-melanoma skin cancer models, including squamous cell carcinoma (HSC-5)
- Supporting stromal cells: dermal fibroblasts (HS68, Detroit 551) and endothelial cells (HMEC-1) for co-culture studies
Molecular & Functional Characterization
- Melanoma lines profiled for key mutations (BRAF, NRAS, NF1) and immunotherapy markers (PD-L1)
- Characterized for metastatic potential, invasion, and angiogenic properties
- Suitable for 2D, 3D spheroid, and complex co-culture models with fibroblasts and endothelial cells
Quality-Certified for Reproducible Research
- STR-authenticated to ensure genetic identity and prevent cross-contamination
- Rigorously tested and certified mycoplasma-free
- Supplied with detailed culture protocols and growth characteristics for reliable experimentation
FAQ
Which cell lines are suitable for studying melanoma metastasis or invasion?
Lines like WM-266-4 and the MeCo/MEGR/MEMOR series are derived from metastatic lesions (e.g., lymph nodes, subcutaneous sites) and are typically more aggressive and invasive. They are excellent models for studying the mechanisms of melanoma dissemination and for testing anti-metastatic compounds.
Can the HMEC-1 microvascular endothelial cell line be used in angiogenesis or transmigration assays?
Yes, absolutely. HMEC-1 is a widely used model of human microvascular endothelial cells. It is particularly valuable for studying tumor-induced angiogenesis, endothelial barrier function, and the extravasation (transmigration) of cancer cells in models of metastasis, which is a critical step in melanoma spread.
What is the BRAF mutation status of the melanoma cell lines?
Mutation status varies. For example, WM-266-4 is a BRAF V600D mutant, while SK-MEL-2 is an NRAS mutant and BRAF wild-type. It is crucial to select a model with the correct genotype for your study, especially for research targeting specific pathways (e.g., BRAF inhibitor studies require a BRAF-mutant line). We provide available mutation data for each cell line.
How are these cell lines authenticated and quality-controlled?
All cell lines, including tumor and normal stromal cells, undergo STR (Short Tandem Repeat) profiling for unique genetic identification. This is essential to prevent misidentification, especially as some lines (like certain fibroblasts) can overgrow others in culture. They are also certified mycoplasma-free.
What are the recommended culture conditions for the HSC-5 squamous cell carcinoma line?
HSC-5 is typically cultured in DMEM supplemented with 10% fetal bovine serum (FBS). It is an adherent cell line. For specific subculturing ratios, recommended trypsinization protocols, and any special medium additives, please refer to the detailed culture protocol provided with the cell line.