- Specification
- Background
- Scientific Data
- Q & A
- Customer Review
The HEK293T cell line is a highly optimized derivative of the human embryonic kidney (HEK293) lineage, engineered through the stable integration of a temperature-sensitive variant of the Simian Virus 40 (SV40) Large T antigen. This genetic modification enables the episomal replication of transfected plasmids containing the SV40 origin of replication, significantly amplifying transient gene expression. Today, HEK293T stands as the industry standard for viral vector production and high-level recombinant protein expression.
- Superior Viral Titers: HEK293T is the "gold standard" host for the packaging of lentiviral and retroviral vectors. Its high transfection efficiency and ability to support massive episomal replication ensure significantly higher viral titers compared to standard HEK293 or other mammalian lines, accelerating timelines for gene therapy and CAR-T research.
- Exceptional Transfection Efficiency: These cells are remarkably amenable to a wide array of transfection methodologies, including calcium phosphate, lipofection, and electroporation. This robustness minimizes optimization efforts and ensures high-yield protein or viral production even with complex, multi-plasmid systems.
- Scalable Growth & Rapid Doubling Time: Characterized by vigorous growth kinetics and adaptation to both adherent and suspension culture formats, HEK293T cells are easily scalable. This makes them ideal for transitioning from bench-top discovery to industrial-scale bioproduction.
- Validated Performance in High-Throughput Screening (HTS): Due to their consistent response and ease of handling, HEK293T cells are a preferred platform for functional genomics, GPCR signaling assays, and drug-target validation studies.
Our HEK293T cells undergo stringent quality control, including authentication via STR profiling and rigorous screening for mycoplasma and adventitious agents. By providing a reliable, high-performance cellular factory, this product serves as an indispensable asset for biopharmaceutical companies and research institutions focused on viral vector engineering, vaccine development, and systemic protein production.
Optimization of Protein UFMylation Modification Method and Its Application in Substrate Identification in Human Embryonic Kidney 293T (HEK293T) Cells
UFMylation plays an essential role in regulating intracellular physiological and pathological processes. Accumulating evidence has demonstrated that dysregulation of UFMylation is closely associated with the progression of various diseases, including cancers and developmental disorders. However, efficient and specific methods for detecting UFMylated substrate proteins remain challenging.
To address these limitations, we developed a systematic strategy to improve UFMylation detection and enable large-scale identification of substrates. In this study, we generated UFSP2 single-KO (UFSP2KO) and UFSP1/UFSP2 double-KO (UFSP1KO/UFSP2KO, DKO) human embryonic kidney 293T (HEK293T) cell lines, capitalizing on the role of UFSPs in UFM1 recycling to increase substrate modification levels. We subsequently optimized the transfection system through single-factor experiments, which revealed that exogenous expression of the E3 ligase components, UFL1 and DDRGK1, rather than the E1 enzyme UBA5 or the E2 enzyme UFC1, is critical for maximizing UFMylation efficiency. Most importantly, by integrating this optimized cellular system with a newly developed antibody specifically recognizing the UFM1 remnant motif (K-ε-Gly-Val [GV]), we achieved robust enrichment of UFMylated peptides and identified more than 600 UFMylation substrates using LC-MS/MS. Furthermore, we validated novel substrates, analyzed amino acid preferences surrounding UFMylation sites, and performed subcellular localization and functional annotation of the identified proteins.
Ask a Question
Write your own review
- You May Also Need
Description: Renal glomerular endothelial cells (GEC) are a specialized microvascular cell type involved in the regulation of glomerular ultrafiltration. They form the inner part of the filtration barrier and are ...
Description: Human Kidney Endothelial Cells from Creative Bioarray are isolated from human kidney tissue. Human Kidney Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based ...
Description: Human Proximal Tubular Epithelial Cells from Creative Bioarray are isolated from normal human proximal tubular tissue. Human Proximal Tubular Epithelial Cells are grown in T25 tissue culture flasks ...
Description: Primary Human Glomerular Microvascular Endothelial Cells were initiated by decapsulated glomeruli isolated from normal human kidney cortical tissue.These cells were originated using Complete ...
Description: Human Kidney Carcinoma Epithelial Cells from Creative Bioarray are isolated from human kidney tumor tissue. Human Kidney Carcinoma Epithelial Cells are grown in T25 tissue culture flasks pre-coated ...
Description: VE-Cad-GFP Expressing Human Glomerular Microvascular Endothelial Cells (VE-Cad-GFP HGMvECs) provided by Creative Bioarray are puromycin-selected from Human Glomerular Microvascular Endothelial Cells ...