Pristane Induced Model
Pristane is an isoprenoid alkane found at high concentration in plants, shark liver and mineral oil. Pristane induced model using B6 or Balb/c mice usually recapitulate clinical features of systemic lupus erythematosus, including high expression of anti-ribonucleic acid protein, anti-DNA and anti-histone autoantibodies and immune complex deposition. In addition, pristane-induced lupus is more pronounced in females than that in male. Creative Bioarray focuses on drug research and development services and helps customers test the efficacy of drug candidates targeting SLE and elucidate the pathogenesis by this model.
Our capabilities
- We detect autoantibodies in animal serum by ELISA, Immunofluorescence, etc.
- We evaluate various cytokines through IHC, ELISA, etc.
- We identify immunocyte subsets in spleens by flow cytometry.
Assays available
- PK/PD blood collections
- Quantitative detection of Anti-dsDNA and total IgG by ELISA
- Clinical chemistry analysis
- Histopathological evaluation
- Cytokine/chemokine analysis
- Flow cytometry in lymph nodes, spleen or bone marrow
- Organ weights (kidneys, spleen, lymph nodes)
- Survival rate
With extensive experience in the field of SLE, we are confident to help you to overcome any upcoming challenges. Our experts are fully capable of customizing our protocols and assays to meet your specific needs. With our help, we wish to facilitate your research with high efficiency.
Study examples
Figure. 1. PD ameliorated lupus manifestations in the kidneys in Pristane induced lupus mouse model (PIL mouse model). PIL mice were treated with vehicle or with PD (45 mg/kg, daily), CYC (1.8 mg/mouse, weekly), MMF (100 mg/kg, daily) for 16 weeks. A The proteinuria was assessed as described in Materials and Methods. B The levels of anti-dsDNA antibodies and anti-Sm antibodies were examined by ELISA. C On the left, representative H&E staining of glomerular and renal vascular lesions in kidneys was shown (× 400). On the right, Austin scores of kidneys were shown. D On the left, the glomeruli were stained for IgG deposition and the representative staining images were shown (× 400). On the right, 25 glomeruli were analyzed and the average score was calculated for each kidney as described in Materials and Methods.
Quotation and ordering
If you have any special needs or questions regarding our services, please feel free to contact us. We look forward to cooperating with you in the future.
Reference
Liao P et al. Polydatin effectively attenuates disease activity in lupus-prone mouse models by blocking ROS-mediated NET formation[J]. Arthritis research & therapy, 2018, 20(1).
