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TF-1

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Cat.No.
CSC-C0395
Description
The TF-1 cell line has been established by T. Kitamura in October 1987 from a heparinized bone marrow aspiration sample from a 35 year old Japanese male with severe pancytopenia. They dont respond to interleukin 5 (IL-5), but to a variety of other lymphokines and cytokines such as interleukin 1 (IL-1), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 9 (IL-9), Interleukin 11 (IL-11), interleukin 13 (IL-13), stem cell factor (SCF), leukemia inhibitory factor (LIF) and nerve growth factor (NGF). The morphological and cytochemical features, and the constitutive expression of globin genes, indicate the commitment of the cells to the erythroid lineage. TPA induces a dramatic differentiation into macrophage-like cells; Hemin and delta-aminolevulinic acid induce hemoglobin synthesis.
Organism
Homo sapiens ,human
STR DNA Profile
Amelogenin: X,Y
CSF1PO:13,13
D13S317: 8,9
D16S539:9,12
D5S818:13
D7S820:12
TH01:7,9
TPOX:8
vWA:15,17
D3S1358: 15
D21S11: 30
D18S51: 13
Penta E: 5,17
Penta D: 10,13
D8S1179: 11,15
FGA: 18,19
Source
bone marrow
Morphology
lymphoblast
Recommended Medium
RPMI-1640 medium supplemented with 1 to 5 ng/ml GM-CSF and 10% h.i. FBS [for long term culture, TF-1 cells need interleukin 3 (IL-3, GM-CSF) in the culture medium].
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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