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TF-1
- Specification
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Cat.No.
CSC-C0395
Description
The TF-1 cell line has been established by T. Kitamura in October 1987 from a heparinized bone marrow aspiration sample from a 35 year old Japanese male with severe pancytopenia. They dont respond to interleukin 5 (IL-5), but to a variety of other lymphokines and cytokines such as interleukin 1 (IL-1), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 9 (IL-9), Interleukin 11 (IL-11), interleukin 13 (IL-13), stem cell factor (SCF), leukemia inhibitory factor (LIF) and nerve growth factor (NGF). The morphological and cytochemical features, and the constitutive expression of globin genes, indicate the commitment of the cells to the erythroid lineage. TPA induces a dramatic differentiation into macrophage-like cells; Hemin and delta-aminolevulinic acid induce hemoglobin synthesis.
Species
Homo sapiens ,human
Source
bone marrow
Recommended Medium
Morphology
lymphoblast
STR DNA Profile
Amelogenin: X,Y
CSF1PO:13,13
D13S317: 8,9
D16S539:9,12
D5S818:13
D7S820:12
TH01:7,9
TPOX:8
vWA:15,17
D3S1358: 15
D21S11: 30
D18S51: 13
Penta E: 5,17
Penta D: 10,13
D8S1179: 11,15
FGA: 18,19
CSF1PO:13,13
D13S317: 8,9
D16S539:9,12
D5S818:13
D7S820:12
TH01:7,9
TPOX:8
vWA:15,17
D3S1358: 15
D21S11: 30
D18S51: 13
Penta E: 5,17
Penta D: 10,13
D8S1179: 11,15
FGA: 18,19
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
How do dyes stain cells?
A:
Permeable nucleic acid stains that can penetrate cell membranes can be used to stain both living and fixed cells without the need for permeabilization. On the other hand, impermeable dyes selectively stain dead cells with disrupted membranes, but not viable cells.
Average Rating: 5.0 | 1 Scientist has reviewed this product
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Creative Bioarray's products have proven to be reliable and consistent in scientific research.
07 Nov 2022
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