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32D

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Cat.No.
CSC-C1101
Description
Established from long-term bone marrow cultures of C3H/HeJ mice infected with the Friend murine leukemia virus; cells are constitutively growth factor-dependent
Organism
Mouse
Karyotype
Murine hypodiploid karyotype - 35(34-35)<2n> - carries 5 centric fusion markers
Source
bone marrow
Culture Properties
suspension
Morphology
mostly round cells growing singly or in small clusters in suspension, some cells are loosely adherent
Recommended Medium
90% RPMI-1640 + 10% h.i. FBS+10U/ml rmIL-3
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD11b +, F4/80 (+)
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 8 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6256W
CSC-6264W
CSC-6284W
CSC-6285W
CSC-6286W
CSC-6287W
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