Cell Transplantation Analysis (FISH)
Cell transplantation was pioneered in the 1950s using haematopoietic stem cells to repopulate the bone marrow in patients with cancers of blood and bone marrow. In animal models, hepatocytes derived from stem cells can be engrafted into the damage liver, and lineage-specific stem cells can repair damaged cornea. Recently more transplantation studies are performed. To date a number of cell types have been shown to successfully engraft into the myocardium, including fetal, neonatal, and embryonic stem cell-derived cardiomyocytes. Fluorescence in situ hybridization (FISH) provides a reliable tool for charaterizing the fate and location of cells in transplantation experiments. Apart from that, the FISH detection can unravel cell fusion phenomena, which could account for apparent transdifferentiation events.
With many years of experience in Fluorescence in situ hybridization (FISH), Creative Bioarray can provide the detection of transplanted cells in transplantation studies.
Figure 1. FISH analysis on brain sections of a male Thy1-YFP mouse shows the co-expression of Thy1-YFP(green signal) in neurons and Cy3-Y-chromosome (red dot) in nuclei counterstained with DAPI.
Figure 2. Detection of fused cells in female-cell-transplanted mouse liver; A: Y paint probe (Red); B: X paint probe; C: Merge
- Allogeneic transplantation detection
For allogeneic transplants, XY chromosome FISH probes are used for detection of transplanted cells. We prefer to detect Y-positive male donoe cells.
- Heterologous transplantation detection
Using our species specific FISH probes, we can detect transplanted cells in heterologous transplants. FISH signals are big and bright, so that transplanted cells can be seen in low magnification.
- Accurate - In Situ Detection Service - Custom design your probe
- Value - We focus on the quality of our service and all supported by competitive pricing
- Efficiency - We are able to provide the fastest turnaround time of any supplier in the industry
Creative Bioarray offers custom Cell Transplantation Analysis services for your scientific research as follows:
- Probe design
- Probe synthesis
- Sample (Cells/FFPE/Others) preparation
- Data analysis
- Donadoni, Chiara, et al. "Improvement of combined FISH and immunofluorescence to trace the fate of somatic stem cells after transplantation." Journal of Histochemistry & Cytochemistry 52.10 (2004): 1333-1339.
- Robinson, Stephen P., et al. "Long-term outcome analysis of reduced-intensity allogeneic stem cell transplantation in patients with mantle cell lymphoma: a retrospective study from the EBMT Lymphoma Working Party." Bone marrow transplantation (2018): 1.
- Allard, Justine, et al. "Immunohistochemical toolkit for tracking and quantifying xenotransplanted human stem cells." Regenerative medicine 9.4 (2014): 437-452.
Quotation and ordering
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For research use only. Not for any other purpose.