Orthotopic Kidney Transplantation Model
Creative Bioarray has successfully established the orthotopic kidney transplantation model, a sophisticated and highly specialized procedure that reflects our advanced capabilities in the field of transplantation research. This model is designed to closely mimic clinical kidney transplantation scenarios, providing an invaluable tool for studying immune responses, graft rejection, and the efficacy of immunosuppressive drugs. Our team of experts has honed the surgical techniques and post-operative care protocols to ensure high survival rates and optimal graft function, making it an ideal platform for translational research. We invite you to explore how our expertise in this model can contribute to your research objectives.
Creative Bioarray's Orthotopic Kidney Transplantation Model
Animal Species
Male, SD Rat
Surgical Procedures
- Step 1: Donor Surgery
An incision is made with a cruciate cut to access the abdominal cavity. Carefully, the renal artery and vein of both the right and left kidneys are dissected, and the adrenal artery and vein are transected using electric coagulation. The kidney and ureter are mobilized, with the ureter being sectioned at the lower pole of the kidney while preserving the surrounding adipose tissue. Administer heparin sodium via the tail vein for systemic anticoagulation 15 minutes prior to the excision of the kidney. The left renal artery and vein are subsequently clamped at their roots and transected. The renal arterial adventitia is then lifted, and a perfusion cannula is inserted for renal preservation.
- Step 2: Recipient Preparation
Following successful anesthesia, the abdominal area is disinfected, and a longitudinal incision is made approximately 1 cm to the left of the midline. The muscle layers are incised using scissors, and self-made retractors are employed to adequately expose the left side of the abdominal cavity. Careful retraction is performed to push the intestinal loops to the right, maximizing exposure of the abdominal contents. Using micro-forceps, the renal artery and vein of the recipient are dissected and temporarily clamped to occlude renal blood flow. The ureter's upper segment is dissected, and the recipient's left kidney is excised following transection of the ureter while absorbing residual blood with a dry cotton ball.
- Step 3: Transplantation Surgery
The donor kidney is then introduced into the recipient's abdominal cavity. End-to-end anastomoses of the renal artery and vein are performed sequentially; upon releasing the clamps, the kidney transitions from a dull yellow to a vibrant red, indicating re-establishment of blood flow. After verifying the absence of bleeding, a urine duct reconstruction is achieved by connecting the ureters of the donor and recipient. The abdominal incision is closed in layers, and antibiotics are administered to prevent postoperative infection.
Applications
- Mechanistic studies of graft rejection
- Preclinical evaluation of immunosuppressive drugs
- Biomarker discovery and immune monitoring
- Graft tolerance research and post-transplant survival analysis
Quotation and Ordering
Creative Bioarray is a leading provider of customized disease model services, offering end-to-end support for drug discovery and preclinical research. Our models are meticulously designed to meet evolving research needs and regulatory expectations.
Feel free to contact us or submit an inquiry today. Our scientific team will assist you in designing a tailored study protocol aligned with your research goals and regulatory strategy.