Endotoxin Induced Uveitis (EIU)

Endotoxin induced uveitis (EIU) is an established animal model that resembles acute anterior uveitis in human. It is induced by systemic injection of LPS, a component located on the outer membranes of gram-negative bacteria. The pathological characteristics of endotoxin- induced uveitis include ocular infiltration of inflammatory cells and leakage of protein into the anterior chamber of the eye. Creative Bioarray focuses on drug research and development services and helps customers explore basic mechanism of uveitis and investigate new potential treatment targeting uveitis by EIU model.

Application

• Acute anterior uveitis

Our capabilities

• We evaluate the severity of uveitis using clinical score system.
• We assess the eye lesions by fundoscopy.
• We isolate and identify T cell subsets from eye or other organs via FACS.

Assays available

• Histopathological evaluation
• Protein concentration in the aqueous humor
• Mediator concentration (prostaglandin, leukotriene, etc.)
• Fundoscopy
• FACS analysis

With extensive experience in the field of uveitis, we are confident to help you to overcome any upcoming challenges. Our experts are fully capable of customizing our protocols and assays to meet your specific needs. With our help, we wish to facilitate your research with high efficiency.

Study examples

Figure. 1. (a) Occlusion of pupil and fibrinous membrane (arrow) was observed in the EIU group at 24 h after LPS injection. (b) Only blood vessel dilatation in the iris (arrow) could be found in the ET group at 24 h after LPS injection. (c) No ocular inflammatory signs were observed in the normal control group.

Figure. 2. (a) In the EIU group, the iris stroma was thickened, and vasodilatation was observed. Fibrin exudations and a large number of inflammatory cell infiltrations of massive neutrophil and small amounts of mononuclear cells and lymphocyte could be observed in the anterior chamber and the vitreous humor of the rats 24 h after LPS immunization. (b) The inflammation levels of the rats in the ET group were significantly decreased compared with those in the EIU group and only a small amount of inflammatory cell infiltration and were found in the iris–ciliary body complex. (c) No inflammatory signs were observed in the normal control group.

Quotation and ordering

If you have any special needs or questions regarding our services, please feel free to contact us at 631-626-9181 or info@creative-bioarray.com. We look forward to cooperating with you in the future.

Reference

1. Yu S, et al. Protection of Lipopolysaccharide (LPS) Preconditioning against Endotoxin-Induced Uveitis (EIU) in Rats Is Associated with Overexpression of Interleukin-1 Receptor-Associated Kinase M(IRAK-M)[J]. Ocular Immunology and Inflammation, 2017:1-8.