TXM13
Cat.No.: CSC-C9741L
Species: Homo sapiens (Human)
Source: Brain Metastasis
Culture Properties: monolayer
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vWA: 16,17
FGA: 22.2,25
Amelogenin: X
TH01: 8
TPOX: 10,11
CSF1P0: 11
D5S818: 12
D13S317: 8,12
D7S820: 8
Shipping Condition: Room Temperature
The TXM-13 (also called TXM13) is a human melanotic melanoma cell line, which was established from a brain metastatic lesion of a melanoma patient. It is a highly tumorigenic and metastatic model, which is widely used in cancer studies. TXM-13 is characterized by its high rate of stable melanin pigmentation. TXM-13 can be maintained in pigmented state with a high efficiency unlike other pigmented melanoma lines (e.g. G361 or SK-MEL-28) that are prone to spontaneous depigmentation during long-term culturing. This stability is explained by the consistent copy number of the tyrosinase gene locus (Chr 11q21). Morphological observation revealed that the cells had a heterogeneous mixture of pigmented and non-pigmented adherent growth. They are usually cultured in a nutrient-rich basal medium supplemented with serum under conventional conditions (37°C, 5% CO2).
TXM-13 displays a stable melanogenic activity and can be used as a tool for researching tyrosinase-mediated melanogenesis, screening depigmenting/whitening drugs, and investigating the pathophysiology of melanoma metastasis, especially to brain.
Tyrosinase-Mediated TXM13 Pigmentation During Long-Term Culture
Melanoma exhibits heterogeneous melanin production, yet its molecular determinants remain unclear. Yin's team characterized TXM13 cells, a melanotic melanoma line that displays heterogeneous pigmentation during subculture (Fig. 1A). To isolate highly pigmented cells, they performed single-cell cloning. Cloned cells maintained robust pigmentation upon scale-up (Fig. 1B) and were harvested for analysis.
Tyrosinase activity assays confirmed that the cloned TXM13 cells exhibited specific DOPA oxidase activity in a concentration-dependent manner (Fig. 1C). This demonstrates that the high pigmentation phenotype is directly associated with functional tyrosinase expression, establishing this clonal line as a model for studying melanin regulation.
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