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LCL-WEI

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Cat.No.
CSC-C0332
Description
Epstein-Barr virus-positive cell line established from peripheral blood in 1977 from male patient with melanoma
Organism
Human
Karyotype
Human near-tetraploid karyotype with diploid (36%) sideline and 4% polyploidy - 90(88-94)<4n>XX/XXY/XXYY - no consistent structural aberrations
Source
B lymphoblastoid cells
Culture Properties
suspension
Morphology
lymphoblastoid cells growing singly and in large clumps in suspension
Recommended Medium
80-90% RPMI-1640 + 10-20% h.i. FBS
Quality Control
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD37 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -/+, sm
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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CONTACT US USA
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Tel: 1-631-626-9181
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Europe
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