IPC-298

Anti-Melanoma Effects of Ni/Cu-NCPs

Bimetallic nanoparticles (NPs) offer enhanced properties for cancer therapy. Huang's team synthesized nickel/copper NPs using the aqueous extract of Artemisia absinthium, a medicinal plant with known antioxidant and anticancer properties. The NPs were characterized using FT-IR, XRD, and FE-SEM. Their antioxidant activity was tested against DPPH, and their anti-melanoma efficacy was evaluated using an MTT assay on HT144, IPC-298, and SKMEL24 cell lines.

In cytotoxicity tests, introducing Ni/Cu-NCPs to melanoma cells (HT144, IPC-298, and SKMEL24) notably reduced ATP levels, causing mitochondrial damage. Ni/Cu-NCPs can produce substantial ROS, enhancing cytotoxicity. They applied Ni/CuNPs@ A. absinthium to cells at 0-1000 μg/mL. Using the MTT test, formazan crystal formation indicated cell viability. Figure 1 shows cytotoxic effects on HT144, IPC-298, and SKMEL24 cell lines, revealing decreased viability with increased Ni/CuNPs loads. Normal cell line HUVEC showed no significant viability drop. Figure 1 illustrates IC₅₀ values for cancer studies as 150, 170, and 147 μg/mL against HT144, IPC-298, and SKMEL24, respectively, highlighting Ni/CuNPs@ A. absinthium's strong antiproliferative effect on melanoma cells, especially in SKMEL24 cells with the lowest IC₅₀ value.

Fig. 1. The efficacy of Ni/CuNPs@ A. absinthium on the cell viability (%) of HUVEC, HT144, IPC-298, and SKMEL24 cell lines (Huang A, Zhang L, et al., 2025).

Anti-Human Melanoma and Antioxidant Properties of Fe₃O₄@CML/Au NPs

Gold nanoparticles (Au NPs) are particularly important for catalysis and medical therapies due to their stability and biocompatibility. However, their small size often leads to aggregation, reducing their potential. This study synthesizes Au NPs immobilized on Fe₃O₄ nanoparticles using carboxymethyl lignin (CML) as a green reducing and stabilizing agent. The nanocomposite was characterized using various physicochemical methods and tested for catalytic reduction of nitroaromatics and anti-human melanoma efficacy.

To measure the anti-human melanoma efficacy of Fe₃O₄@CML/Au NPs nanocomposite, MTT assay was applied on HT144, MUM2C, IPC-298 and SKMEL24 cell lines. Fe₃O₄@CML/Au NPs nanocomposite had high anti-human melanoma efficacy on above tumor cells. The IC50 of Fe₃O₄@CML/Au NPs nanocomposite was 137, 145, 185, and 125 μg/mL against HT144, MUM2C, IPC-298 and SKMEL24 cells, respectively. The best finding of anti-human melanoma properties of Fe₃O₄@CML/Au NPs nanocomposite was seen in the case of the SKMEL24 cell line (Fig. 2). The Fe₃O₄@CML/Au NPs nanocomposite increased significantly the apoptosis and ROS levels in the HT144, MUM2C, IPC-298 and SKMEL24 cells. The data of Fig. 2, Fig. 3 protects the anti-melanoma effects of the Fe₃O₄@CML/Au NPs nanocomposite.

Fig. 2. The properties of Fe₃O₄@CML/Au NPs nanocomposite on the cell viability (%) of HT144, MUM2C, IPC-298 and SKMEL24 cells (Zhao Z J, Wu D J, et al., 2024).

Fig. 3. The properties of Fe₃O₄@CML/Au NPs nanocomposite on the ROS (a.u.) and apoptosis (%) of HT144, MUM2C, IPC-298 and SKMEL24 cells (Zhao Z J, Wu D J, et al., 2024).