Established from the primary (achromic) cutaneous tumor (left thigh) of a 26-year-old man with malignant melanoma (primary tumor histology: SSM level IV); same patient as cell line IGR-37; described as tumorigenic in nude mice
Human flat-moded tetra-/pentaploid karyotype - 104-112<4n>XXY - rearrangements include der(1)t(1;?21)(p13;q11), der(16)t(1;16)(p13;q23) also present in the autologous sister line IGR-37
adherent epithelial-like cells growing in monolayers
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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