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Immortalized Rat Steroidogenic Granulosa Cells stably expressing FSH Receptor (GFSHR-17)
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Cat.No.
CSC-I9001L
Description
Follicle stimulating hormone (FSH) receptor, a receptor uniquely expressed in ovarian granulosa and testicular sertoli cells, play an important role in the regulation of oogenesis, spermatogenesis and steroid hormone production. Unfortunately FSH receptor expression is often lost within 1-2 days ofin vitroculture of primary cells. The Immortalized Rat Steroidogenic Granulosa Cells stably expressing FSH Receptor is responsive to FSH stimulation and produces progesterone as a result at levels comparable to primary cells. This cell line is useful in studying FSH-FSH receptor interactions, as well as how FSH receptor regulates the development and maturation of ovarian follicle. Together with theImmortalized Rat Steroidogenic Granulosa Cells expressing LH Receptor, researchers can compare the LH and FSH signaling in homologues cell systems.
Source
Ovary
Culture Properties
Adherent
Immortalization Method
Serial passaging and calcium phosphate mediated cotransfection by SV40 DNA and Ha-ras oncogene
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
1) Phenotypic response to FSH was characterized by shape changes (cells rounding up) ;
2) FSH receptor expression was measured by radiolabelled 125I-oFSH binding assay- the cells express 27,000 FSH receptors/cell with a Kd of 100-115 pM, similar to the native receptor;
3) Production of progesterone was determined by radioimmunoassay (RIA) ;
4) Production of cAMP was measured by a protein binding method
2) FSH receptor expression was measured by radiolabelled 125I-oFSH binding assay- the cells express 27,000 FSH receptors/cell with a Kd of 100-115 pM, similar to the native receptor;
3) Production of progesterone was determined by radioimmunoassay (RIA) ;
4) Production of cAMP was measured by a protein binding method
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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