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Immortalized Mouse Atrioventricular Cushion Mesenchymal Cells (tsA58-AVM)
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Cat.No.
CSC-I9355L
Description
The Immortalized Mouse Atrioventricular Cushion Mesenchymal Cells (tsA58-AVM) were derived from atrioventricular (AV) cushions of H-2Kb -tsA58 embryos at E9.5 and were conditionally immortalized in subsequent cultures. The immortalized cells display the same characteristics when compared to the parental cells. They express genes that are known to be present in cushion mesenchymal cells, including Bmp2, Bmp4, Cspg2, Has2, Id1, Id3, PlxnA1, Postn, SMA, Snai1, Snai2, Sox9, Tbx20, Twist1, and Vimentin. In the Immortalized Mouse Atrioventricular Cushion Mesenchymal Cells (tsA58-AVM), the expression of Sema6D is upregulated by bone morphogenetic protein (BMP) stimulation. Sema6D is required for BMP-upregulated migration of tsA58-AVM cells. This immortalized cell line is useful in studies focusing on cardiology, particularly in atrioventricular cushion development in mice and the effects ofSema6Dgene.
Species
Mus musculus
Source
Heart
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Conditional immortalization achieved in subsequent cultures as temperature sensitive SV40T antigen mutant gene (tsA58) expression driven by the c-interferon inducible promoter of the H-2Kb gene.
Markers
cTnt, MHC, PECAM, NFACT1, SMA, Vimentin
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Quality Control
1) Immunostaining analysis;
2) Microarray analysis;
3) RT-PCR analysis.
2) Microarray analysis;
3) RT-PCR analysis.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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