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Immortalized Mouse Natural Killer Cells (KIL C.2)
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Cat.No.
CSC-I9259L
Description
A clonal derivative from the CD3-NK1.1+NK cells developed from cocultures of OP-9S/LMJSN and post-5-FU bone marrow MNCs, the Immortalized Mouse Natural Killer Cells (KIL C.2) are dependent on IL-7 for proliferation and survival. Without culturing the cells in IL-7 the cells will undergo apoptosis within 24 hours. The cells are positive for CD43, CD51, CD94, and NKG2D surface markers which are comparable to the parental cells. If cultured with IL-2, the cells terminally differentiate exhibiting large azurophilic granules, cytoplasmic vacuoles, ruffled cell membranes, upregulation in granzyme B, and eventually die off in culture. It is recommended for use in studies relating to natural killer cell development.
Species
Mus musculus
Source
Blood
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Spontaneous immortalization and subcloning by limiting dilution
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Quality Control
1) Flow cytometry analysis for cell surface markers;
2) Wright-Giemsa stain to visualize cells;
3) Western blost used to detect granzyme B.
2) Wright-Giemsa stain to visualize cells;
3) Western blost used to detect granzyme B.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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