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Immortalized Mouse Kupffer Cells (ImKC)
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Cat.No.
CSC-I9264L
Description
Immortalized Mouse Kupffer Cells (ImKC) are isolated from male H-2Kb-tsA58 transgenic mice which expresses the thermolabile mutant tsA58 of SV40 40 large T antigen and were sorted through identifying the F4/80 marker. The immortalized cells expresses p53 levels with increases telomerase activity and retains the some functional features compared to the primary cells. ImKC cells can propagate without the presence of IFNy at 37°C, and are able to produce proinflammatory cytokines when induced with lipopolysaccharide (LPS). These cells are valuable for studies in proinflammatory cytokines (TNF-α, IL-6 and IL-1β) and their roles in Kuppfer cells, in processes in liver injury, and for research in chronic diseases.
Species
Mus musculus
Source
Liver
Culture Properties
Adherent
Morphology
Macrophage-like
Immortalization Method
Isolated from transgenic mice expressing thermolabile mutant tsA58 of SV40 40 large T antigen
Markers
F4/80, CD11b, CD14, and TLR4
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Flow cytometry utilized to analyze cell surface markers F4/80, CD11b, CD14, and TLR4;
2) Western blot analysis on p53 expression;
3) qRT-PCR used to analyze level of LPS induced cytokine production.
2) Western blot analysis on p53 expression;
3) qRT-PCR used to analyze level of LPS induced cytokine production.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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